SIRT7 active protein

Active SIRT7, human recombinant

Cat. Num. AAA845084

• Gene Names: SIRT7; SIR2L7
• Applications: SDS-Page
• Purity: >=85%
• Synonyms: SIRT7; Active SIRT7; human recombinant; NAD-dependent deacetylase 7; SIR2-like protein 7; Sirtuin 7; SIR2L7; Silent Information Regulator 7; SIRT7 active protein

• Host: E Coli
• Purity/Purification: >=85%
• Form/Format: 2 mg/ml in 50 mM Tris, pH 8.0, 100 mM NaCl, containing 5 mM DTT and 20% glycerol. ; Appearance: Liquid
• Concentration: 2 mg/ml (varies by lot)
• Sequence Length: 400

• Applicable Applications for SIRT7 active protein: SDS-PAGE; HPLC
• Activity (Specifications/test method): MyBioSource's Active His-tagged SIRT7 has been tested for its activity using Sirtuin Activity Assay Kit (K324-100). It has a specific activity of >1 uU/mg.
• Biological Activity: >1 uU/mg
• Results: >1 uU/mg
• Unit Definition: One unit of Sirtuin activity is the amount of enzyme that deacetylates an acetylated p53-AFC substrate to finally yield 1 umol of AFC/min at 37 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 6 months

Testing Data

Related Product Information for SIRT7 active protein

Background: The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. There are seven human sirtuins, which have been designated SIRT1-SIRT7. Recently, SIRT7 has been shown to activate transcription by RNA polymerase I and deacetylate p53. SIRT7 prevents progressive functional deterioration of the heart, and is suggested to play an important role in regulation of stress responses and cell death in the heart.

Product Categories/Family for SIRT7 active protein

Epigenetics; Eraser Enzymes; Sirtuins (SIRTs); EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 7706712
• NCBI GeneID: 51547
• NCBI Accession #: NP_057622.1
• NCBI GenBank Nucleotide #: NM_016538.2
• UniProt Accession #: Q9NRC8; Q3MIK4; Q9NSZ6; Q9NUS6; A8K2K0; B3KSU8
• Molecular Weight: 50.1 kDa (2-400 aa + N-terminal polyhistidine tag).
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-7
• NCBI Official Synonym Full Names: sirtuin 7
• NCBI Official Symbol: SIRT7
• NCBI Official Synonym Symbols: SIR2L7
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-7
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-7
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT7
• UniProt Synonym Gene Names: SIR2L7
• UniProt Entry Name: SIR7_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRT7: NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; Nucleolus; EC 3.5.1.-

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleolus organizer region; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; metal ion binding; chromatin binding

Biological Process: activation of transcription on exit from mitosis; negative regulation of transcription from RNA polymerase II promoter; rRNA transcription