Shp-2 active protein

Shp-2 (human), (recombinant)

Cat. Num. AAA566606

• Gene Names: PTPN11; CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3
• Purity: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Synonyms: Shp-2; Shp-2 (human); (recombinant); Shp-2 active protein

• Host: E Coli
• Purity/Purification: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Form/Format: Liquid. In 10mM MES, pH5.7, containing 100mM NaCl, 5mM DTT and 33% glycerol

• Source Note: Full length human Shp-2 (aa 1-593)
• Specific Activity: >=100pmol/min/ug assayed by p-nitrophenylphosphate (pNPP, 50mM) hydrolysis at pH7.0, 30 degree C
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; After initial defrosting, aliquot product into individual tubes and snap freeze, for example in liquid nitrogen or dry ice/ethanol; Avoid freeze/thaw cycles; Shipping: Shipped on Dry Ice

Related Product Information for Shp-2 active protein

Shp-2 is one of two human Src Homology-2 (SH2) containing phosphatases, enzymes in which two regulatory phosphotyrosine binding domains (N-SH2 & C-SH2) lie N-terminal to the catalytic (PTP) domain. In most cases, full activation of the Erk/MAP kinase pathway by receptor tyrosine kinase signaling requires Shp-2, which is thought to act somewhere upstream of Ras.

Product Categories/Family for Shp-2 active protein

Cellular Analysis; Cell Signaling; Proteostasis; Posttranslational Modifications

NCBI and Uniprot Product Information

• NCBI GI #: 33356177
• NCBI GeneID: 5781
• NCBI Accession #: NP_002825.3
• NCBI GenBank Nucleotide #: NM_002834.3
• UniProt Accession #: Q06124; Q96HD7; A8K1D9
• Molecular Weight: 68,436 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 11 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 11
• NCBI Official Symbol: PTPN11
• NCBI Official Synonym Symbols: CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 11; PTP-2C; protein-tyrosine phosphatase 1D; protein-tyrosine phosphatase 2C
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 11
• UniProt Gene Name: PTPN11
• UniProt Synonym Gene Names: PTP2C; SHPTP2; PTP-1D; PTP-2C; SHP-2; Shp2
• UniProt Entry Name: PTN11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: protein complex; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein domain specific binding; protein binding; phospholipase binding; SH3/SH2 adaptor activity; peptide hormone receptor binding; insulin receptor substrate binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; phosphoprotein phosphatase activity; D1 dopamine receptor binding; insulin receptor binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; hormone-mediated signaling; activation of MAPK activity; heart development; platelet-derived growth factor receptor signaling pathway; positive regulation of mitotic cell cycle; reproductive process in a multicellular organism; glucose homeostasis; regulation of protein export from nucleus; negative regulation of insulin secretion; regulation of cell adhesion mediated by integrin; ephrin receptor signaling pathway; abortive mitotic cell cycle; inner ear development; negative regulation of cell adhesion mediated by integrin; negative regulation of growth hormone secretion; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of hormone secretion; regulation of multicellular organism growth; negative regulation of cortisol secretion; organ growth; genitalia development; triacylglycerol metabolic process; DNA damage checkpoint; T cell costimulation; insulin receptor signaling pathway; innate immune response; hormone metabolic process; brain development; platelet formation; blood coagulation; leukocyte migration

Disease: Metachondromatosis; Noonan Syndrome 1; Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1