SCF active protein

Human SCF

Cat. Num. AAA695860

• Gene Names: KITLG; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
• Purity: ≥ 98% as determined by SDS-PAGE Analysis.
• Synonyms: SCF; Human SCF; Recombinant Human SCF; Stem Cell Factor; c-Kit Ligand; Mast Cell Growth Factor (MGF); Steel Factor; SCF active protein

• Host: E Coli
• Purity/Purification: ≥ 98% as determined by SDS-PAGE Analysis.
• Form/Format: Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Acetic Acid.
• Sequence: MEGICRNRV TNNVKDVTK LVANLPKDY MITLKYVPG MDVLPSHCW ISEMVVQLS DSLTDLLDK FSNISEGLS NYSIIDKLV NIVDDLVEC VKENSSKDL KKSFKSPEP RLFTPEEFF RIFNRSIDA FKDFVVASE TSDCVVSST LSPEKDSRV SVTKPFMLP PVA

• Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
• Cell Type: Stem Cells
• Endotoxin: Endotoxin level if <0.1 ng/ug of protein (<1EU/ug).
• Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.
• Reconstitution: Centrifuge before opening. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up one week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: Store at -20°C.

Testing Data

Related Product Information for SCF active protein

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

Product Categories/Family for SCF active protein

Stem Cell Research; Proliferation and Cell Cycle

NCBI and Uniprot Product Information

• NCBI GI #: 134289
• NCBI GeneID: 4254
• UniProt Accession #: P21583; Q16487; Q68DZ2; Q7M4N8; Q9UQK7; A0AV09; A8K2Q4; B7ZLM4
• NCBI Official Full Name: Kit ligand
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: KITLG
• NCBI Official Synonym Symbols: SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
• NCBI Protein Information: kit ligand; c-Kit ligand; steel factor; stem cell factor; mast cell growth factor; familial progressive hyperpigmentation 2
• UniProt Protein Name: Kit ligand
• Protein Family: SCF-associated factor
• UniProt Gene Name: KITLG
• UniProt Synonym Gene Names: MGF; SCF; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_HUMAN

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: extracellular space; cytoskeleton; cytoplasm; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; embryonic hemopoiesis; germ cell programmed cell death; male gonad development; signal transduction; positive regulation of MAP kinase activity; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mast cell apoptosis; positive regulation of melanocyte differentiation; innate immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication

Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7