SCF active protein

Recombinant Human SCF

Cat. Num. AAA4159707

• Gene Names: KITLG; SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69
• Purity: >95% as determined by SDS-PAGE
• Synonyms: SCF; Recombinant Human SCF; Kit ligand; Mast cell growth factor; MGF; c-Kit Ligand; KITLG; SCF active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in Tris and NaCl
• Sequence Length: 273

• Bioactivity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells was found to be <= 0.1 ng/mL
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for SCF active protein

SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotent stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when co-stimulated with other cytokines. The receptor for SCF, designated SCFR (CD117) is the oncogene designated as KIT. The biological activities of SCF are considerably synergised by colony stimulating factors; GM-CSF and G-CSF, and by IL7, EPO and some other growth and differentiation factors. In combination with IL7, SCF stimulates the proliferation of pre-B cells. SCF is also a potent chemoattractant for cells expressing the kit receptor. Recombinant Human SCF is a monomeric protein that contains intra-chain disulfide bonds.

NCBI and Uniprot Product Information

• NCBI GI #: 4505175
• NCBI GeneID: 4254
• NCBI Accession #: NP_000890.1
• NCBI GenBank Nucleotide #: NP_000890.1
• UniProt Accession #: P21583
• Molecular Weight: 18.7 kDa
• NCBI Official Full Name: kit ligand isoform b
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: KITLG
• NCBI Official Synonym Symbols: SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69
• NCBI Protein Information: kit ligand
• UniProt Protein Name: Kit ligand
• Protein Family: SCF-associated factor
• UniProt Gene Name: KITLG
• UniProt Synonym Gene Names: MGF; SCF; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_HUMAN

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]