RANTES Active Protein | CCL5 active protein

RANTES, Recombinant, Human (Regulated on Activation, Normal T-cell Expressed and Secreted)

Cat. Num. AAA650433

• Gene Names: CCL5; SISd; SCYA5; RANTES; TCP228; D17S136E; MGC17164
• Purity: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: RANTES; Recombinant; Human (Regulated on Activation; Normal T-cell Expressed and Secreted); CCL5 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O, carrier protein to 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Tyr-Ser-Ser.

• Biological Activity: ED50 Range = 5.0-2.0ng/ml, determined by the dose dependent chemotaxis of THP-1 cells.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile ddH2O, carrier protein. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CCL5 active protein

Recombinant Human Rantes produced in E.Coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7809.2 Dalton. Recombinant Human Rantes is purified by proprietary chromatographic techniques.

Product Categories/Family for CCL5 active protein

Growth Factors; Cytokines; Growth Factors-RANTES

NCBI and Uniprot Product Information

• NCBI GI #: 2905632
• NCBI GeneID: 6352
• UniProt Accession #: P13501; O43646; Q0QVW8; Q4ZGJ1; Q9NYA2; Q9UBG2; Q9UC99; Q9UBL2
• Molecular Weight: 7.81kD
• NCBI Official Full Name: RANTES
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: CCL5
• NCBI Official Synonym Symbols: SISd; SCYA5; RANTES; TCP228; D17S136E; MGC17164
• NCBI Protein Information: C-C motif chemokine 5; eoCP; SIS-delta; OTTHUMP00000163880; OTTHUMP00000197106; beta-chemokine RANTES; small-inducible cytokine A5; T-cell specific protein p288; t cell-specific protein P228; T-cell-specific protein RANTES; eosinophil chemotactic cytokine; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; regulated upon activation, normally T-expressed, and presumably secreted
• UniProt Protein Name: C-C motif chemokine 5
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL5
• UniProt Synonym Gene Names: D17S136E; SCYA5
• UniProt Entry Name: CCL5_HUMAN

NCBI Description

This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. [provided by RefSeq]

Uniprot Description

Function: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. Ref.3 Ref.8 Ref.10 Ref.11 Ref.12

Subcellular location: Secreted.

Tissue specificity: T-cell and macrophage specific.

Induction: By mitogens.

Post-translational modification: N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in Ref.8. They are assigned by similarity.

Polymorphism: The variant Phe-24 is an antagonist of the chemokine receptors CCR1 and CCR3.

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Mass spectrometry: Molecular mass is 7515±1 Da from positions 27 - 91. Determined by SELDI. Ref.12Molecular mass is 7862.8±1.1 Da from positions 24 - 91. Determined by ESI. Ref.8Molecular mass is 8355±10 Da from positions 24 - 91. Determined by ESI. O-glycosylated. Ref.8