Western Blot (WB)
(Western blot analysis of 721-B lysate using SMNDC1 antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit SMNDC1 Polyclonal Antibody | anti-SMNDC1 antibody
SMNDC1 Antibody
Gene Names
SMNDC1; SMNR; SPF30; TDRD16C
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SMNDC1; Polyclonal Antibody; SMNDC1 Antibody; 30 kDa splicing factor SMNrp; MGC106917; MGC112663; SMN related protein; SMN-related protein; smndc1; SMNR; SPF30; SPF30_HUMAN; Splicing factor 30; survival of motor neuron-related; Survival motor neuron domain containing 1; Survival motor neuron domain containing protein 1; Survival motor neuron domain-containing protein 1; Survival of motor neuron related splicing factor 30; Survival of motor neuron-related-splicing factor 30; anti-SMNDC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SMNDC1 antibody detects endogenous levels of total SMNDC1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
238
Applicable Applications for anti-SMNDC1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human SMNDC1
Subcellular Location
Nucleus Speckle. Nucleus > Cajal Body. Detected in nuclear speckles containing snRNP and in cajal (coiled) bodies.
Tissue Specificity
Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Rabbit (91%), Dog (100%), Chicken (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of 721-B lysate using SMNDC1 antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of 721-B lysate using SMNDC1 antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9606781 at 1/100 staining Mouse pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9606781 at 1/100 staining Mouse pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-SMNDC1 antibody
Description: SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (Survival Motor Neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.
Function: Necessary for spliceosome assembly. Overexpression causes apoptosis.
Subunit Structure: Associates with spliceosomes. Associates with U4/U5/U6 tri-snRNP and with U2 snRNP.
Similarity: The Tudor domain mediates association with dimethylarginines, which are common in snRNP proteins. Belongs to the SMN family.
Function: Necessary for spliceosome assembly. Overexpression causes apoptosis.
Subunit Structure: Associates with spliceosomes. Associates with U4/U5/U6 tri-snRNP and with U2 snRNP.
Similarity: The Tudor domain mediates association with dimethylarginines, which are common in snRNP proteins. Belongs to the SMN family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 27 kDa
Predicted: 27 kDa
Predicted: 27 kDa
NCBI Official Full Name
survival of motor neuron-related-splicing factor 30
NCBI Official Synonym Full Names
survival motor neuron domain containing 1
NCBI Official Symbol
SMNDC1
NCBI Official Synonym Symbols
SMNR; SPF30; TDRD16C
NCBI Protein Information
survival of motor neuron-related-splicing factor 30
UniProt Protein Name
Survival of motor neuron-related-splicing factor 30
Protein Family
UniProt Gene Name
SMNDC1
UniProt Synonym Gene Names
SMNR; SPF30
NCBI Description
This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Necessary for spliceosome assembly. Overexpression causes apoptosis.
Research Articles on SMNDC1
Similar Products
Product Notes
The SMNDC1 smndc1 (Catalog #AAA9606781) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMNDC1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMNDC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SMNDC1 smndc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMNDC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
