Rabbit anti-Human TCEB1 Polyclonal Antibody | anti-TCEB1 antibody

TCEB1, CT (TCEB1, Transcription elongation factor B polypeptide 1, Elongin 15kD subunit, Elongin-C, RNA polymerase II transcription factor SIII subunit C, SIII p15) (MaxLight 550)

Cat. Num. AAA6354399

• Gene Names: ELOC; SIII; TCEB1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TCEB1; Polyclonal Antibody; CT (TCEB1; Transcription elongation factor B polypeptide 1; Elongin 15kD subunit; Elongin-C; RNA polymerase II transcription factor SIII subunit C; SIII p15) (MaxLight 550); SIII p15; anti-TCEB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TCEB. Species Sequence Homology: bovine, mouse and rat
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-TCEB1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 69-98 in the C-terminal region of human TCEB1, UniProt Accession #NP_005639.1; Q15369; P83941; P83940; Q2KII4.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS644796. This demonstrates the TCEB1 antibody detected the TCEB1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma using MBS644796 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS644796 for immunohistochemistry.)

Related Product Information for anti-TCEB1 antibody

This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Product Categories/Family for anti-TCEB1 antibody

Antibodies; Transcription Factors

References

1. Marcsisin, S.R., et al. J. Mol. Biol. 402(5):892-904 (2010). 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 3. Lievens, S., et al. J. Proteome Res. 8(2):877-886 (2009). 4. Jalava, S.E., et al. Int. J. Cancer 124(1):95-102 (2009). 5. Piessevaux, J., et al. J. Biol. Chem. 283(31):21334-21346 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5032161
• NCBI GeneID: 6921
• NCBI Accession #: NP_005639.1
• NCBI GenBank Nucleotide #: NM_005648.3
• UniProt Accession #: Q15369; P83941; P83940; Q2KII4
• Molecular Weight: 10,832 Da
• NCBI Official Full Name: elongin-C isoform a
• NCBI Official Synonym Full Names: elongin C
• NCBI Official Symbol: ELOC
• NCBI Official Synonym Symbols: SIII; TCEB1
• NCBI Protein Information: elongin-C
• UniProt Protein Name: Transcription elongation factor B polypeptide 1
• Protein Family: Transcription elongation factor B polypeptide
• UniProt Gene Name: TCEB1
• UniProt Synonym Gene Names: EloC
• UniProt Entry Name: ELOC_HUMAN

NCBI Description

This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

TCEB1: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). Belongs to the SKP1 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: nucleoplasm; VCB complex; cytosol

Molecular Function: protein binding; protein complex binding

Biological Process: transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; regulation of transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; positive regulation of RNA elongation from RNA polymerase II promoter; RNA elongation from RNA polymerase II promoter; gene expression