RanBP2 Delta Active Protein | RanBP2 active protein
RanBP2 Delta (human), (recombinant) (GST) (FG Fragment)
Gene Names
RANBP2; ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358
Purity
>=90% (SDS-PAGE)Purified by GST-based chromatography
Synonyms
RanBP2 Delta; RanBP2 Delta (human); (recombinant) (GST) (FG Fragment); RanBP2 active protein
Host
E Coli
Purity/Purification
>=90% (SDS-PAGE)
Purified by GST-based chromatography
Purified by GST-based chromatography
Form/Format
Liquid. In 20mM HEPES, pH7.3 containing 110mM potassium acetate, 2mM magnesium acetate, 0.8mM EGTA, 1mM DTT
Source Note
RanBP2deltaFG (aa 2553-2838) is fused to a GST-tag
Biological Activity
The enzyme facilitates in vitro SUMOylation using SUMO-1, SUMO E1, and SUMO E2 UbcH9
Preparation and Storage
For long term storage, store at -80 degree C
Shipping: Shipped on Dry Ice
Shipping: Shipped on Dry Ice
Related Product Information for RanBP2 active protein
Post-translational modification with SUMO-1 regulates protein-protein interactions, localisation and stability. SUMOylation requires the E1 enzyme Aos1/Uba2 and the E2 enzyme Ubc9. A family of E3-like factors, PIAS proteins has been discovered, however, the nucleoporin RanBP2 also has SUMO-1 E3-like activity.
Modification of the histone deactylase HDAC4 depends upon the presence of an intact nuclear localisation signal and is catalysed by the nuclear pore complex (NPC) RanBP2 protein. This suggests that SUMOylation of HDAC4 takes place at the NPC and is coupled to nuclear import. This and other findings suggests that SUMOylation may be an important regulatory mechanism for the
control of transcriptional repression mediated by both class I and II HDACs.
Mdm2, a ubiquitin ligase that acts on the tumour suppressor p53, is regulated by SUMOylation. It has been shown that Mdm2 is SUMOylated during nuclear translocation by RanBP2 and then further SUMOylated once in the nucleus by PIASxbeta and PIAS1.
The RanGTPase activating protein RanGAP1 has essential functions in both nucleocytoplasmic transport and mitosis. In interphase, a significant fraction of vertebrate SUMO-1-modified RanGAP1 forms a stable complex with RanBP2 at nuclear pore complexes. RanGAP1 is subject to phosphorylation and it has been demonstrated that phosphorylated RanGAP1 remains associated with RanBP2 and Ubc9 in mitosis. Thus, mitotic phosphorylation may have functional consequences for the RanGTPase cycle and/or for RanBP2-dependent SUMOylation.
Modification of the histone deactylase HDAC4 depends upon the presence of an intact nuclear localisation signal and is catalysed by the nuclear pore complex (NPC) RanBP2 protein. This suggests that SUMOylation of HDAC4 takes place at the NPC and is coupled to nuclear import. This and other findings suggests that SUMOylation may be an important regulatory mechanism for the
control of transcriptional repression mediated by both class I and II HDACs.
Mdm2, a ubiquitin ligase that acts on the tumour suppressor p53, is regulated by SUMOylation. It has been shown that Mdm2 is SUMOylated during nuclear translocation by RanBP2 and then further SUMOylated once in the nucleus by PIASxbeta and PIAS1.
The RanGTPase activating protein RanGAP1 has essential functions in both nucleocytoplasmic transport and mitosis. In interphase, a significant fraction of vertebrate SUMO-1-modified RanGAP1 forms a stable complex with RanBP2 at nuclear pore complexes. RanGAP1 is subject to phosphorylation and it has been demonstrated that phosphorylated RanGAP1 remains associated with RanBP2 and Ubc9 in mitosis. Thus, mitotic phosphorylation may have functional consequences for the RanGTPase cycle and/or for RanBP2-dependent SUMOylation.
Product Categories/Family for RanBP2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
358,199 Da
NCBI Official Full Name
E3 SUMO-protein ligase RanBP2
NCBI Official Synonym Full Names
RAN binding protein 2
NCBI Official Symbol
RANBP2
NCBI Official Synonym Symbols
ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358
NCBI Protein Information
E3 SUMO-protein ligase RanBP2; P270; nucleoporin 358; nucleoporin Nup358; 358 kDa nucleoporin; ran-binding protein 2; transformation-related protein 2; nuclear pore complex protein Nup358; acute necrotizing encephalopathy 1 (autosomal dominant)
UniProt Protein Name
E3 SUMO-protein ligase RanBP2
Protein Family
UniProt Gene Name
RANBP2
UniProt Synonym Gene Names
NUP358; RanBP2
UniProt Entry Name
RBP2_HUMAN
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Product Notes
The RanBP2 ranbp2 (Catalog #AAA566734) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RanBP2 Delta, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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