Rabbit anti-Human, Mouse MAP3K11 Polyclonal Antibody | anti-MAP3K11 antibody

MAP3K11 (MAP3K11, MLK3, PTK1, SPRK, Mitogen-activated protein kinase kinase kinase 11, Mixed lineage kinase 3, Src-homology 3 domain-containing proline-rich kinase) (Azide free) (HRP)

Cat. Num. AAA6318576

• Gene Names: MAP3K11; MLK3; PTK1; SPRK; MLK-3; MEKK11
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAP3K11; Polyclonal Antibody; MAP3K11 (MAP3K11; MLK3; PTK1; SPRK; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase) (Azide free) (HRP); Src-homology 3 domain-containing proline-rich kinase; anti-MAP3K11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MAP3K11 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MAP3K11 antibody is generated from rabbits immunized with MAP3K11 recombinant protein.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAP3K11 Antibody in mouse lung tissue lysates (35ug/lane).MAP3K11 (arrow) was detected using the purified Pab.)

Related Product Information for anti-MAP3K11 antibody

MAP3K11 is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42.

Product Categories/Family for anti-MAP3K11 antibody

Antibodies; Protein Kinases

References

Rangasamy, V., et al. Cancer Res. 70(4):1731-1740(2010). Velho, S., et al. Hum. Mol. Genet. 19(4):697-706(2010). Cole, E.T., et al. Biochim. Biophys. Acta 1793(12):1811-1818(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 71153819
• NCBI GeneID: 4296
• UniProt Accession #: Q16584; Q53H00; Q59F06; Q6P2G4; B4DS76
• Molecular Weight: 65,344 Da
• NCBI Official Full Name: Mitogen-activated protein kinase kinase kinase 11
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Symbol: MAP3K11
• NCBI Official Synonym Symbols: MLK3; PTK1; SPRK; MLK-3; MEKK11
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 11
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 11
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K11
• UniProt Entry Name: M3K11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]

Uniprot Description

MLK3: a TKL kinase of the MLK family. Has an amino-terminal SH3 domain followed by the kinase domain, two leucine zipper domains, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. Phosphorylates IkappaB and activates SEK1 and MKK7.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 11q13.1-q13.3

Cellular Component: centrosome; cytoplasm; membrane; microtubule

Molecular Function: ATP binding; identical protein binding; JUN kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; mitogen-activated protein kinase kinase kinase binding; protein binding; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity; Rac GTPase binding

Biological Process: activation of JNK activity; activation of JNKK activity; activation of MAPK activity; cell death; cell proliferation; JNK cascade; microtubule-based process; peptidyl-tyrosine phosphorylation; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of neuron apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation