Prolactin Active Protein | PRL active protein

Prolactin, Recombinant, Human (LTH, Luteotropic Hormone, Lactogenic Hormone)

Cat. Num. AAA635539

• Purity: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: Prolactin; Recombinant; Human (LTH; Luteotropic Hormone; Lactogenic Hormone); PRL active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a lyophilized powder in 10mM sodium phosphate, pH 8.0, 50mM sodium chloride.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ile-Cys.

• Biological Activity: Prolactin is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be < 0.065ng/ml.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PRL active protein

Prolactin is a lactogenic hormone secreted by the adenohypophysis. Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation. Prolactin has been shown also to have cytokine-like activities and to have important immunoregulatory activities. It contributes to the development of lymphoid tissues and the maintenance of physiological immune function and also modulates a variety of T-cell immune responses. Prolactin has been reported to activate cellular proliferation in nonreproductive tissue, such as liver, spleen, and thymus. It induces significant proliferation in aortic smooth muscle cells and also enhances proliferation of these cells induced by PDGF. Prolactin also appears to be directly mitogenic for pancreatic beta cells. Prolactin is also mitogenic for cultured astrocytes.

Recombinant Human Prolactin is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of ~23kD.

Product Categories/Family for PRL active protein

Molecular Biology; MB-Hormones; Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 254675133
• NCBI GeneID: 5617
• NCBI Accession #: NP_001157030.1
• NCBI GenBank Nucleotide #: NM_001163558.2
• UniProt Accession #: P01236; Q15199; Q92996
• Molecular Weight: ~23kD
• NCBI Official Full Name: prolactin
• NCBI Official Synonym Full Names: prolactin
• NCBI Official Symbol: PRL
• NCBI Protein Information: prolactin; decidual prolactin
• UniProt Protein Name: Prolactin
• Protein Family: Prolactin
• UniProt Gene Name: PRL
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PRL_HUMAN

NCBI Description

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extracellular region

Molecular Function: protein binding; prolactin receptor binding; hormone activity

Biological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; positive regulation of JAK-STAT cascade; regulation of multicellular organism growth; female pregnancy