PPP1CA active protein

PPP1CA, 1-330aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA204483

• Gene Names: PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: PPP1CA; 1-330aa; Human; His tag; E Coli (Bioactivity Validated); Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; PP-1A; PP1alpha; PPP1A; PPP1CA active protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. 50mM Tris-HCl (pH 8.5), 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50%glycerol
• Concentration: 0.2 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK
• Sequence Length: 330

• Applicable Applications for PPP1CA active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
• Assay: 1. Prepare a 200ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP.; 2. Pre-warm reaction mix in heat block at 37C; 3. Add recombinant PPP1CA solution with various concentrations (2ug, 3ug) in 200ul reaction buffer.; 4. Mix by inversion and incubate at 37C for 10minutes.; 5. Stop the reaction by adding the 800ul of 0.1N NaOH.; 6. Record the absorbance at 405nm
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PPP1CA active protein

PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CA protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for PPP1CA active protein

Enzymes & Proteases

References

He B., et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:843-848.; Trinkle-Mulcahy L., et al. (2001) J. Cell Sci. 114:4219-4228.

NCBI and Uniprot Product Information

• NCBI GI #: 4506003
• NCBI GeneID: 5499
• NCBI Accession #: NP_002699
• NCBI GenBank Nucleotide #: NM_002708.3
• UniProt Accession #: P62136; P08129; P20653; P22802; Q07161; A6NNR3; B2R908
• Molecular Weight: 39.7 kDa (350aa), confirmed by MALDI-TOF
• NCBI Official Full Name: serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 1 catalytic subunit alpha
• NCBI Official Symbol: PPP1CA
• NCBI Official Synonym Symbols: PP1A; PP-1A; PPP1A; PP1alpha
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP1CA
• UniProt Synonym Gene Names: PPP1A; PP-1A

NCBI Description

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Dephosphorylates CENPA (PubMed:25556658). Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323).