PGK2 active protein

PGK2, human recombinant

Cat. Num. AAA844966

• Gene Names: PGK2; PGKB; PGKPS; HEL-S-272; dJ417L20.2
• Applications: SDS-Page
• Purity: >=85%
• Synonyms: PGK2; human recombinant; Phosphoglycerate kinase 2; dJ417L20.2; PGKB; PGKPSS; PGK2 active protein

• Host: E Coli
• Purity/Purification: >=85%
• Form/Format: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl and 1 mM DTT; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
• Sequence Length: 417

• Applicable Applications for PGK2 active protein: SDS-PAGE
• Biological Activity: Specific activity is >11 units/mg. One unit will convert 1.0 umole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25 degree C
• Results: Specific activity is >11 units/mg
• Unit Definition: One unit will convert 1.0 umole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25 degree C
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PGK2 active protein

Background: PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Product Categories/Family for PGK2 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 31543397
• NCBI GeneID: 5232
• NCBI Accession #: NP_620061.2
• NCBI GenBank Nucleotide #: NM_138733.4
• UniProt Accession #: P07205; Q9H107; B2R6Y8
• Molecular Weight: 46.9 kDa (437 aa, 1-417 aa + His Tag)
• NCBI Official Full Name: phosphoglycerate kinase 2
• NCBI Official Synonym Full Names: phosphoglycerate kinase 2
• NCBI Official Symbol: PGK2
• NCBI Official Synonym Symbols: PGKB; PGKPS; HEL-S-272; dJ417L20.2
• NCBI Protein Information: phosphoglycerate kinase 2
• UniProt Protein Name: Phosphoglycerate kinase 2
• Protein Family: Phosphoglycerate kinase
• UniProt Gene Name: PGK2
• UniProt Synonym Gene Names: PGKB
• UniProt Entry Name: PGK2_HUMAN

NCBI Description

This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]

Uniprot Description

PGK2: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]

Protein type: EC 2.7.2.3; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: cytoplasm; nucleus

Molecular Function: phosphoglycerate kinase activity; ATP binding

Biological Process: sperm motility; glycolysis; phosphorylation