Western Blot (WB)
(Sample Data: Representative western blot (1:2000) from HeLa cell lysate using a rabbit pAb RSK1 antibody recognized by Anti-Rabbit HRP)
Goat Anti-Rabbit Polyclonal Antibody
Anti-Rabbit, HRP Conjugated
Reactivity
IgG, IgM from Rabbit(Other rabbit immunoglobulins may also be detectable)
Applications
Western Blot, ELISA
Purity
Affinity purified with rabbit immunoglobulins on agarose followed by adsorption with human serum albumin and human gamma globulins
Synonyms
Anti-Rabbit; Polyclonal Antibody; HRP Conjugated; anti-Anti-Rabbit antibody
Host
Goat
Reactivity
IgG, IgM from Rabbit
(Other rabbit immunoglobulins may also be detectable)
(Other rabbit immunoglobulins may also be detectable)
Clonality
Polyclonal
Specificity
Anti-Rabbit HRP conjugated secondary antibody recognizes rabbit IgG and IgM.
Purity/Purification
Affinity purified with rabbit immunoglobulins on agarose followed by adsorption with human serum albumin and human gamma globulins
Form/Format
PBS pH7.2, 50% Glycerol
Applicable Applications for anti-Anti-Rabbit antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 17846:19845-ELISA: 2% skim milk blocking solution is recommended
Immunogen
Affinity purified rabbit immunoglobulins
Conjugation
HRP Conjugated
Preparation and Storage
Store at 4 degree C for several months, and at -20 degree C for longer periods; do not add NaN3 as this will inhibit the HRP enzyme. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot (1:2000) from HeLa cell lysate using a rabbit pAb RSK1 antibody recognized by Anti-Rabbit HRP)
Western Blot (WB)
(Sample Data: Representative western blot (1:2000) from HeLa cell lysate using a rabbit pAb RSK1 antibody recognized by Anti-Rabbit HRP)
Related Product Information for anti-Anti-Rabbit antibody
Affinity purified rabbit immunoglobulins were used to raise goat antibodies specific to the rabbit IgG. The goat immune serum is subsequently affinity purified using immunoglobulins immobilized to agarose. The antibodies are then conjugated to horse radish peroxidase (HRP) by reductive amination in a 2:1 molar ratio. The HRP conjugated secondary antibody can recognize the primary immunoglobulin and give a signal in the presence of a chemiluminescent substrate. The signal can be detected using an electronic imager or autoradiography film (1).
Product Categories/Family for anti-Anti-Rabbit antibody
References
1. Nakane PK, Pierce GB: Enzyme-labeled antibodies: preparation and application for localization of antigens, J Histochem Cytochem 1966, 14:929
Similar Products
Product Notes
The Anti-Rabbit (Catalog #AAA515980) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rabbit, HRP Conjugated reacts with IgG, IgM from Rabbit (Other rabbit immunoglobulins may also be detectable) and may cross-react with other species as described in the data sheet. AAA Biotech's Anti-Rabbit can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 17846:19845-ELISA: 2% skim milk blocking solution is recommended. Researchers should empirically determine the suitability of the Anti-Rabbit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Anti-Rabbit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
