Neurotrophin-4 Active Protein | NTF4 active protein

Recombinant Human Neurotrophin-4 (rHu NT-4)

Cat. Num. AAA546122

• Gene Names: NTF4; NT4; NT5; NT-4; NT-5; NTF5; GLC10; GLC1O; NT-4/5
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Synonyms: Neurotrophin-4; Recombinant Human Neurotrophin-4 (rHu NT-4); human NT-4; Human Neurotropin-4; NTF4 active protein

• Host: E Coli
• Purity/Purification: >97% by SDS-PAGE and HPLC analyses.
• Form/Format: Lyophilized from a 0.2mm filtered concentrated (0.5mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.; Physical Appearance: Sterile FilteredWhite lyophilized (freeze-dried) powder.
• Sequence Length: 210

• Molecular Weight Information: 21.7 kDa, a single, non-glycosylated polypeptide chain containing 187 amino acids.
• Biological Activity: Fully biologically active when compared to standard. Determined by the dosedependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.
• Endotoxin Level: Less than 1EU/ug of rHuNT-4 as determined by LAL method.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.
• Preparation and Storage: This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Related Product Information for NTF4 active protein

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. Human NT-4 shares 48 - 52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 promotes the development and survival of selected peripheral and CNS neurons. NT-4 induced TrkB signaling augments NMDAreceptor activity and increases neuronal sensitivity to excitotoxic cell death. It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle. NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration.

NCBI and Uniprot Product Information

• NCBI GI #: 5453808
• NCBI GeneID: 4909
• NCBI Accession #: NP_006170.1
• NCBI GenBank Nucleotide #: NM_006179.4
• UniProt Accession #: P34130; Q6FH56; AAB20549
• Molecular Weight: 22,427 Da
• NCBI Official Full Name: neurotrophin-4 preproprotein
• NCBI Official Synonym Full Names: neurotrophin 4
• NCBI Official Symbol: NTF4
• NCBI Official Synonym Symbols: NT4; NT5; NT-4; NT-5; NTF5; GLC10; GLC1O; NT-4/5
• NCBI Protein Information: neurotrophin-4; neurotrophin-5; neutrophic factor 4; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 5 (neurotrophin 4/5)
• UniProt Protein Name: Neurotrophin-4
• Protein Family: Neurotrophin
• UniProt Gene Name: NTF4
• UniProt Synonym Gene Names: NTF5; NT-4; NT-5
• UniProt Entry Name: NTF4_HUMAN

NCBI Description

This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq, Jul 2008]

Uniprot Description

NT-4/5: Target-derived survival factor for peripheral sensory sympathetic neurons. Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O). A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Belongs to the NGF-beta family.

Protein type: Secreted; Cytokine; Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: endoplasmic reticulum lumen; cytoplasmic membrane-bound vesicle; extracellular region

Molecular Function: neurotrophin p75 receptor binding; growth factor activity

Biological Process: epidermis development; mechanoreceptor differentiation; long-term memory; cell-cell signaling; adult locomotory behavior; regulation of neuron differentiation; sensory organ boundary specification; ganglion mother cell fate determination; negative regulation of neuron apoptosis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Glaucoma 1, Open Angle, O