Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling PIP4K2A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasmic staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).)
Rabbit anti-Human, Mouse PIP4K2A Polyclonal Antibody | anti-PIP4K2A antibody
PIP4K2A Antibody (C-term)
IHC-P: 1:25
FC/FACS: 1:25
WB: 1:1000
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling PIP4K2A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasmic staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling PIP4K2A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasmic staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. brain section using PIP4K2A Antibody (C-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. brain section using PIP4K2A Antibody (C-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using PIP4K2A Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). It was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using PIP4K2A Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). It was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Western Blot (WB)
(All lanes : Anti-PIP4K2A Antibody (C-term) at 1:1000 dilutionLane 1: human brain lysatesLane 2: Hela whole cell lysatesLane 3: K562 whole cell lysatesLane 4: mouse brain lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PIP4K2A Antibody (C-term) at 1:1000 dilutionLane 1: human brain lysatesLane 2: Hela whole cell lysatesLane 3: K562 whole cell lysatesLane 4: mouse brain lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Background: Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.
NCBI and Uniprot Product Information
NCBI Description
Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008]
Uniprot Description
PIP4K2A: Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.
Protein type: EC 2.7.1.149; Motility/polarity/chemotaxis; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate
Chromosomal Location of Human Ortholog: 10p12.2
Cellular Component: plasma membrane; cytosol; nucleus
Molecular Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; 1-phosphatidylinositol-5-phosphate 4-kinase activity; ATP binding
Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process
Research Articles on PIP4K2A
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Product Notes
The PIP4K2A pip4k2a (Catalog #AAA9232058) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIP4K2A Antibody (C-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PIP4K2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA). IF: 1:25 IHC-P: 1:25 FC/FACS: 1:25 WB: 1:1000. Researchers should empirically determine the suitability of the PIP4K2A pip4k2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIP4K2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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