NAP-2 (PPBP) (CXCL7) Active Protein | NAP-2 active protein

Recombinant Human NAP-2 (PPBP) (CXCL7)

Cat. Num. AAA4159699

• Gene Names: PPBP; PBP; TC1; TC2; TGB; LDGF; MDGF; TGB1; B-TG1; CTAP3; CXCL7; NAP-2; SCYB7; THBGB; LA-PF4; THBGB1; Beta-TG; CTAPIII; CTAP-III
• Purity: >95% as determined by SDS-PAGE
• Synonyms: NAP-2 (PPBP) (CXCL7); Recombinant Human NAP-2 (PPBP) (CXCL7); CXCL7; PBP (parent molecule); CTAP-III (precursor); Platelet basic protein; C-X-C motif chemokine 7; Leukocyte-derived growth factor; LDGF; Macrophage-derived growth factor; MDGF; Small-inducible cytokine B7; SCYB7; TGB1; THBGB1; NAP2; Recombinant Human Neutrophil Activating Peptide-2 (PPBP); NAP-2 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered 20 mM PB,100 mM NaCl solution pH 7.5.
• Sequence Length: 128

• Bioactivity: Determined by its ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Related Product Information for NAP-2 active protein

The chemokine neutrophil-activating peptide 2 (NAP-2) is a 70-amino acid residue polypeptide that is formed from platelet-derived precursors by proteolytic processing. Mature NAP-2 stimulates various effector functions of polymorphonuclear neutrophil granulocytes (PMN) including directed chemotactic migration, exocytosis of lysosomal enzymes and secondary granule contents, and up-regulation of adhesion receptors. NAP-2 has been assigned to a subfamily now termed alpha-chemokines. The alpha-chemokines contain four cysteine residues at highly conserved positions which enclose the core region of the molecules. The first two cysteines are separated by a single amino acid, forming a motif (CXC) that distinguishes the alpha-chemokine from the beta-chemokine subfamily, where these cysteines are in directly adjacent position.

NCBI and Uniprot Product Information

• NCBI GI #: 129874
• NCBI GeneID: 5473
• UniProt Accession #: P02775
• Molecular Weight: 8.0 kDa
• NCBI Official Full Name: Platelet basic protein
• NCBI Official Synonym Full Names: pro-platelet basic protein
• NCBI Official Symbol: PPBP
• NCBI Official Synonym Symbols: PBP; TC1; TC2; TGB; LDGF; MDGF; TGB1; B-TG1; CTAP3; CXCL7; NAP-2; SCYB7; THBGB; LA-PF4; THBGB1; Beta-TG; CTAPIII; CTAP-III
• NCBI Protein Information: platelet basic protein
• UniProt Protein Name: Platelet basic protein
• UniProt Gene Name: PPBP
• UniProt Synonym Gene Names: CTAP3; CXCL7; SCYB7; TGB1; THBGB1; PBP; LDGF; MDGF; CTAP-III; CTAP-III(1-81); Beta-TG; NAP-2(74); NAP-2(73); NAP-2; NAP-2(1-66); NAP-2(1-63)
• UniProt Entry Name: CXCL7_HUMAN

NCBI Description

The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. The protein also is an antimicrobial protein with bactericidal and antifungal activity. [provided by RefSeq, Nov 2014]

Uniprot Description

PPBP: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Chemokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; glucose transmembrane transporter activity

Biological Process: platelet activation; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; glucose transport; leukocyte migration during inflammatory response; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; platelet degranulation; positive regulation of cell division; defense response to bacterium; immune response; inflammatory response; blood coagulation