MMP-8 active protein
MMP-8 (catalytic domain) (human), (recombinant)
Purity
>=90% (SDS-PAGE)Purified by multi-step chromatography
Synonyms
MMP-8; MMP-8 (catalytic domain) (human); (recombinant); MMP-8 active protein
Host
E Coli
Purity/Purification
>=90% (SDS-PAGE)
Purified by multi-step chromatography
Purified by multi-step chromatography
Form/Format
Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20uM ZnCl2, 0.5% Brij-35, and 30% glycerol
Source Note
Active Matrix Metalloproteinase-8 (MMP-8, neutrophil collagenase, collagenase-2) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-8 (Phe99-Gln269) with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-8 which lacks the C-terminal hemopexin domain1. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected
Activity
Preincubation of MMP-8 catalytic domain at 13.6 nM with the broad-spectrum inhibitor GM6001 at 20nM for 1 hour completely inhibits enzymatic activity
Specific Activity
>=150U/ug. One U=100 pmol/min at 37 degree C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100uM) as substrate
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
For long term storage, store at -80 degree C
Shipping: Shipped on Dry Ice
Shipping: Shipped on Dry Ice
Product Categories/Family for MMP-8 active protein
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
203kDa
UniProt Protein Name
Neutrophil collagenase
UniProt Gene Name
MMP8
UniProt Synonym Gene Names
CLG1; MMP-8; PMNL-CL
UniProt Entry Name
MMP8_HUMAN
Uniprot Description
MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.
Protein type: Secreted; EC 3.4.24.34; Protease; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11q22.3
Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region
Molecular Function: zinc ion binding; metalloendopeptidase activity; serine-type endopeptidase activity; calcium ion binding
Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis
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Product Notes
The MMP-8 mmp8 (Catalog #AAA566599) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MMP-8, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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