Bovine Oncostatin M Receptor ELISA Kit | OSMR elisa kit

Bovine Oncostatin M Receptor ELISA Kit

Cat. Num. AAA740816

• Gene Names: OSMR; OSMRB; PLCA1
• Reactivity: Bovine
• Synonyms: Oncostatin M Receptor; Bovine Oncostatin M Receptor ELISA Kit; OSMR elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This OSMR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine OSMR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OSMR elisa kit

Principle of the Assay: OSMR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OSMR antibody and an OSMR-HRP conjugate. The assay sample and buffer are incubated together with OSMR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OSMR concentration since OSMR from samples and OSMR-HRP conjugate compete for the anti-OSMR antibody binding site. Since the number of sites is limited, as more sites are occupied by OSMR from the sample, fewer sites are left to bind OSMR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OSMR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for OSMR elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 115529091
• NCBI GeneID: 9180
• Molecular Weight: 39,504 Da
• NCBI Official Full Name: Oncostatin M receptor
• NCBI Official Synonym Full Names: oncostatin M receptor
• NCBI Official Symbol: OSMR
• NCBI Official Synonym Symbols: OSMRB; PLCA1
• NCBI Protein Information: oncostatin-M-specific receptor subunit beta; IL-31RB; IL-31R-beta; IL-31R subunit beta; IL-31 receptor subunit beta; interleukin-31 receptor subunit beta; oncostatin-M specific receptor beta subunit
• UniProt Protein Name: Oncostatin-M-specific receptor subunit beta
• Protein Family: Oncostatin-M-specific receptor
• UniProt Gene Name: OSMR
• UniProt Synonym Gene Names: OSMRB; IL-31 receptor subunit beta; IL-31R subunit beta; IL-31R-beta; IL-31RB
• UniProt Entry Name: OSMR_HUMAN

NCBI Description

This gene encodes a member of the type I cytokine receptor family. The encoded protein heterodimerizes with interleukin 6 signal transducer to form the type II oncostatin M receptor and with interleukin 31 receptor A to form the interleukin 31 receptor, and thus transduces oncostatin M and interleukin 31 induced signaling events. Mutations in this gene have been associated with familial primary localized cutaneous amyloidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

OSMR: Associates with IL31RA to form the IL31 receptor. Binds IL31 to activate STAT3 and possibly STAT1 and STAT5. Capable of transducing OSM-specific signaling events. Defects in OSMR are the cause of amyloidosis primary localized cutaneous type 1 (PLCA1); also known as familial lichen amyloidosis or familial cutaneous lichen amyloidosis. PLCA1 is a hereditary primary amyloidosis characterized by localized cutaneous amyloid deposition. This condition usually presents with itching (especially on the lower legs) and visible changes of skin hyperpigmentation and thickening (lichenification) that may be exacerbated by chronic scratching and rubbing. The amyloid deposits probably reflect a combination of degenerate keratin filaments, serum amyloid P component, and deposition of immunoglobulins. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: oncostatin-M receptor complex

Molecular Function: oncostatin-M receptor activity; growth factor binding

Biological Process: positive regulation of acute inflammatory response; response to cytokine stimulus; positive regulation of cell proliferation

Disease: Amyloidosis, Primary Localized Cutaneous, 1