Interleukin 18, Rat Active Protein | Il18 active protein
Interleukin 18, Recombinant, Rat (IL-18, IFN-gamma inducing factor, IGIF)
Gene Names
Il18; IL-18
Purity
Highly Purified95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Synonyms
Interleukin 18; Rat; Recombinant; Rat (IL-18; IFN-gamma inducing factor; IGIF); Il18 active protein
Host
E Coli
Purity/Purification
Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form/Format
Supplied as a lyophilized powder in 0.4X PBS, pH 7.2.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be His-Phe-Gly-Arg-Leu.
Biological Activity
Rat IL-18 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IFN-gamma by murine lymph node cells (4 x 105 cells/well) co-stimulated with sub optimal dose of ConA (0.75ug/ml) is 12 ng/ml. The medium was RPMI containing 10% FCS and 50µM 2-ME.
Dimers and Aggregates
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of rat IL-18.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.213 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-18 as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 0.213 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-18 as a Reference Standard.
Solubility
It is recommended to reconstitute the lyophilized IL-18 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
-20 degree C
Stability: Lyophilized Interlekin-18 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-18 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Stability: Lyophilized Interlekin-18 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-18 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Related Product Information for Il18 active protein
Interleukin-18 is a member of the interleukin-1 family of cytokines with pro-inflammatory and tumor-suppressive properties. Its ability to potently enhance the production of interferon-gamma indicates in particular the crucial function of interleukin-18 as an immunomodulatory molecule. In fact, high levels of interleukin-18 are detected in human diseases associated with immunoactivation including viral or bacterial infections and chronic inflammation. Animal models suggest suppression of interleukin-18 bioactivity as a novel therapeutic concept specifically for the treatment of chronic inflammatory diseases such as rheumatoid arthritis, Crohn's disease, and psoriasis. It is the only cytokine with a unique capacity to induce T helper 1 or T helper 2 polarization, depending on the immunologic context.
Recombinant Rat IL-18 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18260 Dalton. Recombinant Rat Interleukin-18 is purified by proprietary chromatographic techniques.
Recombinant Rat IL-18 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18260 Dalton. Recombinant Rat Interleukin-18 is purified by proprietary chromatographic techniques.
Product Categories/Family for Il18 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18.26kD
NCBI Official Full Name
interleukin-18
NCBI Official Synonym Full Names
interleukin 18
NCBI Official Symbol
Il18
NCBI Official Synonym Symbols
IL-18
NCBI Protein Information
interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor
UniProt Protein Name
Interleukin-18
Protein Family
UniProt Gene Name
Il18
UniProt Synonym Gene Names
Igif
UniProt Entry Name
IL18_RAT
NCBI Description
a pro-inflammatory cytokine; acts as a modulator of immune functions [RGD]
Uniprot Description
Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular location: Secreted.
Sequence similarities: Belongs to the IL-1 family.
Research Articles on Il18
1. IL-18 may serve as an additional marker to monitor the severity of inflammation during pancreatitis.
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Product Notes
The Il18 il18 (Catalog #AAA650216) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be His-Phe-Gl y-Arg-Leu. It is sometimes possible for the material contained within the vial of "Interleukin 18, Rat, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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