Interleukin 1 alpha Active Protein | IL1A active protein

Interleukin 1 alpha, Recombinant, Mouse (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)

Cat. Num. AAA650776

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
• Purity: Highly Purified; 98% by SDS-PAGE and HPLC analyses.
• Synonyms: Interleukin 1 alpha; Recombinant; Mouse (IL-1a; Hematopoietin-1; Lymphocyte-activating Factor; LAF; Endogenous Pyrogen; Leukocyte Endogenous Mediator; LEM; Mononuclear Cell Factor; MCF); IL1A active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 98% by SDS-PAGE and HPLC analyses.
• Form/Format: Lyophilized from 1mM Tris buffer solution. May also be supplied as a liquid.

• Biological Activity: Murine IL-1a is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of murine D10S cells is 0.002ng/ml, corresponding to a specific activity of 5x10e8 units/mg.
• Endotoxin Level: 0.1ng/ug
• Reconstitution: Lyophilized murine IL-1a is soluble in water and most aqueous buffers. The lyophilized protein can be reconstituted in water to a concentration of 100ug/ml. This solution can be diluted into other buffered solutions or stored at -20 degree C for future use. For most in vitro applications, IL-1a exerts its biological activity in the concentration range of 0.1 to 10.0 ng/ml.
• Preparation and Storage: -20 degree C; Stability: The lyophilized protein, though stable at room temperature, is best stored desiccated below 0 degree C. Reconstituted murine IL-1a should be stored in working aliquots at -20 degree C.

Related Product Information for IL1A active protein

IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Murine IL-1a is a 17.9kD protein containing 156 amino acid residues.

Product Categories/Family for IL1A active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 27894330
• NCBI GeneID: 3552
• NCBI Accession #: NP_000566.3
• NCBI GenBank Nucleotide #: NM_000575.3
• UniProt Accession #: P01583; Q7RU02; Q53QF9
• Molecular Weight: 17.9kD
• NCBI Official Full Name: interleukin-1 alpha proprotein
• NCBI Official Synonym Full Names: interleukin 1, alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA
• NCBI Protein Information: interleukin-1 alpha; IL-1 alpha; hematopoietin-1; OTTHUMP00000161990; preinterleukin 1 alpha; pro-interleukin-1-alpha
• UniProt Protein Name: Interleukin-1 alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1
• UniProt Entry Name: IL1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq]

Uniprot Description

Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Subunit structure: Monomer.

Subcellular location: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Domain: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.

Sequence similarities: Belongs to the IL-1 family.