IL-28A active protein

Mouse IL-28A

Cat. Num. AAA692521

• Gene Names: Ifnl2; Il28a; IL-28A; EG330496
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Synonyms: IL-28A; Mouse IL-28A; Interferon lambda-2; IFN-lambda-2; Interleukin-28A; Ifnl2; Il28a; EG330496; IL-28A active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEK DMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS A LATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQ EAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV
• Sequence Length: 193

• Species: Mouse
• Biological Activity: The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis(EMC) virus is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.
• Reconstitution: It is recommended to reconstitute the lyophilized IL-28A in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C.

Related Product Information for IL-28A active protein

IL-28A is distantly related to type I interferons and the IL-10 family. Expression of IL-28A is induced by viral infection which interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha. IL-28A exhibits common features with type I IFNs such as antiviral activity, antiproliferative activity and in vivo antitumour activity. IL-28A acts similarly to IFNs, but is less effective generally and has activity in a more limited range of cell lines. IFN-ambda 1, IFN-lambda 2 and IFN-lambda 3 are closely positioned genes on human chromosome 19. IL-28A induces ELR(-) CXC chemokine mRNA in human peripheral blood mononuclear cells, in an IFN-gamma-independent manner. IL-28A is able to generate tolerogenic DCs, an activity that could thwart IFN-beta functions. IL-28A produced in response to viral infection, activates both monocytes and macrophages producing a restricted panel of cytokines and therefore is an important factor in activating innate immune responses at the site of viral infection.

NCBI and Uniprot Product Information

• NCBI GI #: 130494425
• NCBI GeneID: 330496
• NCBI Accession #: NP_001019844.2
• NCBI GenBank Nucleotide #: NM_001024673.2
• UniProt Accession #: Q4VK74; E9Q2S2
• Molecular Weight: 21,740 Da
• NCBI Official Full Name: interferon lambda-2
• NCBI Official Synonym Full Names: interferon lambda 2
• NCBI Official Symbol: Ifnl2
• NCBI Official Synonym Symbols: Il28a; IL-28A; EG330496
• NCBI Protein Information: interferon lambda-2
• UniProt Protein Name: Interferon lambda-2
• UniProt Gene Name: Ifnl2
• UniProt Synonym Gene Names: Il28a; IL-28A

Uniprot Description

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.