Growth Hormone, Chicken Active Protein | GH active protein
Growth Hormone, Chicken, Recombinant
Gene Names
GH; GH1
Purity
Highly Purified98% as determined by:
Synonyms
Growth Hormone; Chicken; Recombinant; GH active protein
Host
E Coli
Purity/Purification
Highly Purified
98% as determined by:
98% as determined by:
Form/Format
Supplied as a lyophilized powder from 0.3% NaHCO3 adjusted to pH 8. Reconstitution: It is recommended to reconstitute the lyophilized cGH in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml and not more than 3mg/ml, which can then b
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.
Biological Activity
chGH is fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.
Dimers and Aggregates
The purified cGH (22K) consists of > 99% monomers as determined by gel-filtration chromatography.
Endotoxin Level
Less than 0.1ng/ug (IEU/ug) of cGH (22 K).
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.75 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of others GHs as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 0.75 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of others GHs as a Reference Standard.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O or buffer of choice and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for GH active protein
Recombinant chicken growth hormone (cGH) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton.
Product Categories/Family for GH active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22.26kD
NCBI Official Full Name
growth hormone
NCBI Official Synonym Full Names
growth hormone
NCBI Official Symbol
GH
NCBI Official Synonym Symbols
GH1
NCBI Protein Information
somatotropin; growth hormone 1; neural retina growth hormone
UniProt Protein Name
Somatotropin
Protein Family
UniProt Gene Name
GH
UniProt Entry Name
SOMA_CHICK
Uniprot Description
Function: Growth hormone plays an important role in growth control.
Subcellular location: Secreted.
Sequence similarities: Belongs to the somatotropin/prolactin family.
Research Articles on GH
Similar Products
Product Notes
The GH gh (Catalog #AAA635534) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Ph e-Pro-Ala. It is sometimes possible for the material contained within the vial of "Growth Hormone, Chicken, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
