Rabbit anti-Human, Mouse TAF2 Polyclonal Antibody | anti-TAF2 antibody

TAF2, CT (TAF2, CIF150, TAF2B, Transcription initiation factor TFIID subunit 2, 150kD cofactor of initiator function, RNA polymerase II TBP-associated factor subunit B, TBP-associated factor 150kD, Transcription initiation factor TFIID 150kD subunit) (APC

Cat. Num. AAA6353370

• Gene Names: TAF2; MRT40; TAF2B; CIF150; TAFII150
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF2; Polyclonal Antibody; CT (TAF2; CIF150; TAF2B; Transcription initiation factor TFIID subunit 2; 150kD cofactor of initiator function; RNA polymerase II TBP-associated factor subunit B; TBP-associated factor 150kD; Transcription initiation factor TFIID 150kD subunit) (APC; Transcription initiation factor TFIID 150kD subunit; anti-TAF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TAF2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TAF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1159~1188 amino acids from the C-terminal region of human TAF2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

((LEFT) Western blot analysis of TAF2 Antibody (C-Term) in MCF-7 cell line lysates (35ug/lane). TAF2 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of TAF2 Antibody (C-Term) in mouse NIH-3T3 cell line lysates (35ug/lane). TAF2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(TAF2 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAF2 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TAF2 Antibody (C-Term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TAF2 antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators.

Product Categories/Family for anti-TAF2 antibody

Antibodies; Transcription Factors

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Kim, J.E., et al. J. Proteome Res. 4(4):1339-1346(2005). Guermah, M., et al. Mol. Cell 12(4):991-1001(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 40674143
• NCBI GeneID: 6873
• UniProt Accession #: Q6P1X5; O43487; O43604; O60668; Q86WW7; Q8IWK4; B2RE82
• Molecular Weight: 136,971 Da
• NCBI Official Full Name: TAF2 protein, partial
• NCBI Official Synonym Full Names: TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa
• NCBI Official Symbol: TAF2
• NCBI Official Synonym Symbols: MRT40; TAF2B; CIF150; TAFII150
• NCBI Protein Information: transcription initiation factor TFIID subunit 2; TAF(II)150; TBP-associated factor 150 kDa; 150 kDa cofactor of initiator function; cofactor of initiator function, 150kD subunit; RNA polymerase II TBP-associated factor subunit B; transcription initiation
• UniProt Protein Name: Transcription initiation factor TFIID subunit 2
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF2
• UniProt Synonym Gene Names: CIF150; TAF2B; TAF(II)150; TAFII-150; TAFII150
• UniProt Entry Name: TAF2_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF2: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter. Belongs to the TAF2 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein binding; zinc ion binding; metallopeptidase activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression; proteolysis; G2/M transition of mitotic cell cycle; response to organic cyclic substance

Disease: Mental Retardation, Autosomal Recessive 40