GPI active protein

GPI, human recombinant

Cat. Num. AAA844992

• Gene Names: GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: GPI; human recombinant; Glucose-6-phosphate isomerase; AMF; GNPI; NLK; PGI; PHI; SA36.; GPI active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1 mM DTT; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAALTRDPQF KLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK FCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT LEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ
• Sequence Length: 558

• Applicable Applications for GPI active protein: SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 mug of protein (determined by LAL method)
• Biological Activity: Specific activity is > 90 units/mg, and one unit will convert 1.0 umole D-fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 25 degree C.
• Results: Specific activity is > 90 units/mg
• Unit Definition: One unit will convert 1.0 umole D-fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 25 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for GPI active protein

Background: Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for GPI active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z) Diabetes; Obesity & Metabolic Syndrome; Signaling Pathways in Diabetes and Obesity; AMPK; Insulin and Leptin Signaling; Proteins/Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 18201905
• NCBI GeneID: 2821
• NCBI Accession #: NP_000166.2
• NCBI GenBank Nucleotide #: NM_000175.3
• UniProt Accession #: P06744; Q9BRD3; Q9BSK5; Q9UHE6; B4DG39
• Molecular Weight: 65.3 kDa (578 aa, 1-558 aa + His Tag)
• NCBI Official Full Name: glucose-6-phosphate isomerase isoform 2
• NCBI Official Synonym Full Names: glucose-6-phosphate isomerase
• NCBI Official Symbol: GPI
• NCBI Official Synonym Symbols: AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
• NCBI Protein Information: glucose-6-phosphate isomerase
• UniProt Protein Name: Glucose-6-phosphate isomerase
• Protein Family: GPI-anchored protein
• UniProt Gene Name: GPI
• UniProt Synonym Gene Names: GPI; AMF; NLK; PGI; PHI; SA-36
• UniProt Entry Name: G6PI_HUMAN

NCBI Description

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Protein type: Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - pentose phosphate pathway; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 5.3.1.9; Cytokine; Apoptosis

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: nucleoplasm; extracellular space; neuron projection; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: monosaccharide binding; glucose-6-phosphate isomerase activity; growth factor activity; cytokine activity; intramolecular transferase activity

Biological Process: aldehyde catabolic process; methylglyoxal biosynthetic process; glycolysis; glucose 6-phosphate metabolic process; glucose metabolic process; pathogenesis; negative regulation of caspase activity; humoral immune response; gluconeogenesis; learning and/or memory; hemostasis; carbohydrate metabolic process; angiogenesis; negative regulation of neuron apoptosis

Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency