Glucose-6-phosphate isomerase Active Protein | GPI active protein

Glucose-6-phosphate isomerase, 1-558aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA206224

• Gene Names: GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Glucose-6-phosphate isomerase; 1-558aa; Human; His tag; E Coli (Bioactivity Validated); GPI; AMF; GNPI; NLK; PGI; PHI; SA36; GPI active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ
• Sequence Length: 558

• Applicable Applications for GPI active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 400 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will convert 1.0 umole of D-Fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GPI active protein

Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GPI active protein

Enzymes & Proteases; ; Angiogenesis; Glycobiology; Metabolism; Neurodisease

References

Lin HY., et al. (2009) Biochim Biophys Acta. 1794 (2):315-23. ; Beutler E., et al. (1997) Blood cells Mol. 23:402-409

NCBI and Uniprot Product Information

• NCBI GI #: 18201905
• NCBI GeneID: 2821
• NCBI Accession #: NP_000166
• NCBI GenBank Nucleotide #: NM_000175.4
• UniProt Accession #: P06744; Q9BRD3; Q9BSK5; Q9UHE6; B4DG39
• Molecular Weight: 65.3kDa (578aa), confirmed by MALDI-TOF
• NCBI Official Full Name: glucose-6-phosphate isomerase isoform 2
• NCBI Official Synonym Full Names: glucose-6-phosphate isomerase
• NCBI Official Symbol: GPI
• NCBI Official Synonym Symbols: AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
• NCBI Protein Information: glucose-6-phosphate isomerase
• UniProt Protein Name: Glucose-6-phosphate isomerase
• Protein Family: Glucose-6-phosphate isomerase
• UniProt Gene Name: GPI
• UniProt Synonym Gene Names: GPI; AMF; NLK; PGI; PHI; SA-36

NCBI Description

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Uniprot Description

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.