Follistatin Active Protein | FST active protein

Follistatin, Recombinant, Human (FST, FS, Activin-binding Protein)

Cat. Num. AAA638167

• Gene Names: FST; FS
• Purity: Highly Purified; ~95% as determined by RP-HPLC and SDS-PAGE
• Synonyms: Follistatin; Recombinant; Human (FST; FS; Activin-binding Protein); FST active protein

• Host: E Coli
• Purity/Purification: Highly Purified; ~95% as determined by RP-HPLC and SDS-PAGE
• Form/Format: Supplied as a lyophilized powder from SBS. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to 0.1mg/ml, which can then be further diluted to other aqueous solutions.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Asn-Cys-Trp-Leu.

• Biological Activity: Recombinant Follistatin is fully biologically active when compared to standard.; The activity is determined by the ability to neutralize Activin A inhibitory effect of mouse MPC-11 cells. The expected ED50 is 0.1-0.4ug/ml in the presence of 7.5ng/ml Activin A.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for FST active protein

Follistatin (FS), a glycoprotein, plays an important role in cell growth and differentiation through the neutralization of the biological activities of activins. Follistatin (FST) is a monomeric activin-binding and neutralization protein that has at least three isoforms in human tissues and fluids. The full-length FS315 protein has an acidic 26-residue C-terminal tail that is not present in the shortest form, FS288, due to alternative splicing. An intermediate form, FS303, was identified in follicular fluid that is presumably derived by proteolytic processing of this tail domain. Interestingly, the biochemistry of each of these three isoforms is distinct, including their ability to bind to cell surface proteoglycans, an activity that ranks in the order FS288 > FS303 > FS315.

Product Categories/Family for FST active protein

Molecular Biology; MB-Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 182721
• NCBI GeneID: 10468
• UniProt Accession #: P19883; Q9BTH0; B5BU94
• Molecular Weight: 31.5kD
• NCBI Official Full Name: follistatin
• NCBI Official Synonym Full Names: follistatin
• NCBI Official Symbol: FST
• NCBI Official Synonym Symbols: FS
• NCBI Protein Information: follistatin; activin-binding protein; follistatin isoform FST317
• UniProt Protein Name: Follistatin
• Protein Family: Follistatin
• UniProt Gene Name: FST
• UniProt Synonym Gene Names: FS
• UniProt Entry Name: FST_HUMAN

NCBI Description

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. [provided by RefSeq, Jul 2008]

Uniprot Description

FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: heparan sulfate proteoglycan binding; signal transducer activity; protein binding; activin binding

Biological Process: BMP signaling pathway; positive regulation of hair follicle development; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; hair follicle morphogenesis; gamete generation; hemopoietic progenitor cell differentiation; keratinocyte proliferation; negative regulation of transcription from RNA polymerase II promoter; skeletal development; pattern specification process; female gonad development; odontogenesis of dentine-containing teeth

Disease: Polycystic Ovary Syndrome 1