Follistatin Active Protein | FST active protein

Follistatin Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120416

• Gene Names: FST; FS
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Follistatin; Follistatin Protein; Human; Recombinant (His Tag); Human Follistatin/FST Protein (His Tag); FS Protein; follistatin; FST active protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp329

• Species: Human
• Activity: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-40ng/mL.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gly 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human FST (NP_037541.1)(Met1-Asp329) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FST active protein

Background: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. follistatin are expressed and subserve local regulatory roles in numerous extragonadal tissues, including brain, adrenal, bone marrow, and placenta but perhaps most notably in anterior pituitary-the classical target tissue for inhibin, the activin-follistatin system may play a key role in early embryogenesis. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone follistatin is a binding protein to activin. Since activin binds to follistatin, it is imperative to determine the nature of the activin/follistatin binding complex.

References

Carroll RS, et al. (1989) Inhibin, activin, and follistatin: regulation of follicle-stimulating hormone messenger ribonucleic acid levels. Mol Endocrinol. 3(12): 1969-76.Fainsod A, et al. (1997) The dorsalizing and neural inducing gene follistatin is an antagonist of BMP-4. Mech Dev. 63(1): 39-50.Kaiser UB, et al. (1992) Follistatin gene expression in the pituitary: localization in gonadotropes and folliculostellate cells in diestrous rats. Endocrinology. 130(5): 3048-56.

NCBI and Uniprot Product Information

• NCBI GI #: 7242222
• NCBI GeneID: 10468
• NCBI Accession #: NP_037541.1
• NCBI GenBank Nucleotide #: NM_013409.2
• UniProt Accession #: P19883; Q9BTH0; B5BU94
• Molecular Weight: 34,803 Da
• NCBI Official Full Name: follistatin isoform FST344
• NCBI Official Synonym Full Names: follistatin
• NCBI Official Symbol: FST
• NCBI Official Synonym Symbols: FS
• NCBI Protein Information: follistatin; activin-binding protein; follistatin isoform FST317
• UniProt Protein Name: Follistatin
• Protein Family: Follistatin
• UniProt Gene Name: FST
• UniProt Synonym Gene Names: FS
• UniProt Entry Name: FST_HUMAN

NCBI Description

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. [provided by RefSeq, Jul 2008]

Uniprot Description

FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: heparan sulfate proteoglycan binding; signal transducer activity; protein binding; activin binding

Biological Process: hair follicle morphogenesis; negative regulation of transcription from RNA polymerase II promoter; keratinocyte proliferation; pattern specification process; odontogenesis of dentine-containing teeth; BMP signaling pathway; positive regulation of hair follicle development; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; gamete generation; hemopoietic progenitor cell differentiation; skeletal development; female gonad development

Disease: Polycystic Ovary Syndrome 1