SDS-Page
(Fig.SDS-PAGE analysis of Human FGF21 protein, His Tag.)
FGF21 active protein
Human FGF21 Protein, His Tag
Purity
>95% as determined by SDS-PAGE
Synonyms
FGF21; Human FGF21 Protein; His Tag; Recombinant Human FGF21 Protein; His Tag (Fibroblast growth factor 21) expressed in E. coli; UNQ3115/ PRO10196; Fibroblast growth factor 21; FGF21 active protein
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from 50 mM Tris, 10 % glycerol, 0.05 % Brig 35, pH 8.0
Sequence
Amino acid sequence derived from mature form of human FGF21 (NP_061986.1) (His 29-Ser 209) was expressed, with a polyhistide tag at the N-terminus.
Sequence Length
209
Species
Human
Protein Length
The recombinant human FGF21 consisting of 188 amino acids and has a calculated molecular mass of 20.2 kDa. It migrates as an 23 kDa band in SDS-PAGE under reducing conditions as predicted.
Activity
Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1-1 ug/mL in the presence of 2.5 ug/mL of rmKlotho beta.
Usage Notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human FGF21 protein, His Tag in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
SDS-Page
(Fig.SDS-PAGE analysis of Human FGF21 protein, His Tag.)
SDS-Page
(Fig.SDS-PAGE analysis of Human FGF21 protein, His Tag.)
Related Product Information for FGF21 active protein
Fibroblast growth factor 21 (FGF21) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 has a hydrophobic amino terminus, which is a typical signal sequence, and appears to be a secreted protein. The metabolic regulator fibroblast growth factor 21 (FGF21) has antidiabetic properties in animal models of diabetes and obesity. FGF21 is a novel adipokine associated with obesity-related metabolic complications in humans. The paradoxical increase of serum FGF21 in obese individuals, which may be explained by a compensatory response or resistance to FGF21, warrants further investigation. FGF-21, which we have identified as a novel metabolic factor, exhibits the therapeutic characteristics necessary for an effective treatment of diabetes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20.2 kDa
NCBI Official Full Name
fibroblast growth factor 21
NCBI Official Synonym Full Names
fibroblast growth factor 21
NCBI Official Symbol
FGF21
NCBI Protein Information
fibroblast growth factor 21
Protein Family
NCBI Description
Theis gene encodes a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. This protein is a secreted endocrine factor that functions as a major metabolic regulator. The encoded protein stimulates the uptake of glucose in adipose tissue. [provided by RefSeq, Mar 2016]
Research Articles on FGF21
Similar Products
Product Notes
The FGF21 (Catalog #AAA9718694) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Amino acid sequence derived from mature form of human FGF21 (NP_061986.1) (His 29-Ser 209) was expressed, with a polyhistid e tag at the N-terminus. It is sometimes possible for the material contained within the vial of "FGF21, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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