FBP1 active protein

FBP1, human recombinant

Cat. Num. AAA844789

• Gene Names: FBP1; FBP
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: FBP1; human recombinant; Fructose-1; 6-bisphosphatase 1; FBPase1; FBP; FBP1 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol and 1 mM DTT.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ
• Sequence Length: 338

• Applicable Applications for FBP1 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 0.30 unit/ml, in which one unit will convert 1.0 umole of fructose 1, 6-diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 25 degree C
• Results: Specific activity is > 0.30 unit/ml
• Unit Definition: One unit will convert 1.0 umole of fructose 1, 6-diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 25 degree C
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for FBP1 active protein

Background: Fructose-1, 6-bisphosphatase 1(FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.

Product Categories/Family for FBP1 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 16579888
• NCBI GeneID: 2203
• NCBI Accession #: NP_000498.2
• NCBI GenBank Nucleotide #: NM_000507.3
• UniProt Accession #: P09467; O75571; Q53F94; Q96E46
• Molecular Weight: 39.0 kDa (358 aa, 1-338 aa + His Tag)
• NCBI Official Full Name: fructose-1,6-bisphosphatase 1
• NCBI Official Synonym Full Names: fructose-1,6-bisphosphatase 1
• NCBI Official Symbol: FBP1
• NCBI Official Synonym Symbols: FBP
• NCBI Protein Information: fructose-1,6-bisphosphatase 1
• UniProt Protein Name: Fructose-1,6-bisphosphatase 1
• Protein Family: Fat-body protein
• UniProt Gene Name: FBP1
• UniProt Synonym Gene Names: FBP; FBPase 1
• UniProt Entry Name: F16P1_HUMAN

NCBI Description

Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq, Jul 2008]

Uniprot Description

FBPase: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans, FBP1 in the liver and FBP2 in muscle. While both forms are inhibited allosterically by AMP, NAD and Ca2+, the muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Deficiency of FBP1 leads to a disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.

Protein type: Carbohydrate Metabolism - fructose and mannose; EC 3.1.3.11; Mitochondrial; Phosphatase (non-protein); Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cytoplasm; cytosol

Molecular Function: monosaccharide binding; identical protein binding; protein binding; metal ion binding; fructose-bisphosphatase activity; AMP binding

Biological Process: dephosphorylation; carbohydrate metabolic process; negative regulation of Ras protein signal transduction; glucose metabolic process; regulation of gluconeogenesis; pathogenesis; negative regulation of cell growth; fructose 6-phosphate metabolic process; negative regulation of glycolysis; protein homotetramerization; gluconeogenesis; fructose metabolic process

Disease: Fructose-1,6-bisphosphatase Deficiency