DLD active protein

DLD, 36-509aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203646

• Gene Names: DLD; E3; LAD; DLDD; DLDH; GCSL; PHE3
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: DLD; 36-509aa; Human; His tag; E Coli (Bioactivity Validated); Dihydrolipoyl dehydrogenase mitochondrial; DLDH; E3; GCSL; LAD; PHE3; Dihydrolipoyl dehydrogenase; mitochondrial Diaphorase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate dehydrogenase; E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex; Glycine cleavage system L protein; Glycine cleavage system protein L; Lipoamide reductase; Lipoyl dehydrogenase; PHE 3; ; DLD active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMADQ PIDADVTVIG SGPGGYVAAI KAAQLGFKTV CIEKNETLGG TCLNVGCIPS KALLNNSHYY HMAHGKDFAS RGIEMSEVRL NLDKMMEQKS TAVKALTGGI AHLFKQNKVV HVNGYGKITG KNQVTATKAD GGTQVIDTKN ILIATGSEVT PFPGITIDED TIVSSTGALS LKKVPEKMVV IGAGVIGVEL GSVWQRLGAD VTAVEFLGHV GGVGIDMEIS KNFQRILQKQ GFKFKLNTKV TGATKKSDGK IDVSIEAASG GKAEVITCDV LLVCIGRRPF TKNLGLEELG IELDPRGRIP VNTRFQTKIP NIYAIGDVVA GPMLAHKAED EGIICVEGMA GGAVHIDYNC VPSVIYTHPE VAWVGKSEEQ LKEEGIEYKV GKFPFAANSR AKTNADTDGM VKILGQKSTD RVLGAHILGP GAGEMVNEAA LALEYGASCE DIARVCHAHP TLSEAFREAN LAASFGKSIN F
• Sequence Length: 509

• Applicable Applications for DLD active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 200 units/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DLD active protein

DLD (Dihydrolipoamide dehydrogenase), also known as GCSL (glycine cleavage system L protein), is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. DLD is a flavin-dependent oxidoreductase and functions as a component of the alpha-keto acid dehydrogenase, the pyruvate dehydrogenase, the alpha-ketoglutarate dehydrogenase, the branched-chain alpha-keto acid dehydrogenase and as the L protein in the mitochondrial glycine cleavage system. Mutations in DLD protein can result in MSuD (maple syrup urine disease) and congenital infantile lactic acidosis. Recombinant human DLD protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for DLD active protein

Metabolism

References

Giannopoulou E., et al. (2009) Anticancer Res. 29 (12):5077-82. ; Daee DL., et al. (2009) Proc Natl Acad Sci u S A. 107 (1):157-62.;

NCBI and Uniprot Product Information

• NCBI GI #: 91199540
• NCBI GeneID: 1738
• NCBI Accession #: NP_000099
• NCBI GenBank Nucleotide #: NM_000108.4
• UniProt Accession #: P09622; Q14131; Q14167; Q59EV8; Q8WTS4; B2R5X0; B4DHG0; B4DT69
• Molecular Weight: 54.4 kDa (511aa)
• NCBI Official Full Name: dihydrolipoyl dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: dihydrolipoamide dehydrogenase
• NCBI Official Symbol: DLD
• NCBI Official Synonym Symbols: E3; LAD; DLDD; DLDH; GCSL; PHE3
• NCBI Protein Information: dihydrolipoyl dehydrogenase, mitochondrial
• UniProt Protein Name: Dihydrolipoyl dehydrogenase, mitochondrial
• Protein Family: Delta-like protein
• UniProt Gene Name: DLD
• UniProt Synonym Gene Names: GCSL; LAD; PHE3

NCBI Description

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). In monomeric form has additional moonlighting function as serine protease (PubMed:17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction ().