Rabbit DLD Polyclonal Antibody | anti-DLD antibody

DLD Antibody

Cat. Num. AAA9403435

• Gene Names: DLD; E3; LAD; DLDD; DLDH; GCSL; PHE3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: DLD; Polyclonal Antibody; DLD Antibody; E3; LAD; DLDD; DLDH; GCSL; anti-DLD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total DLD protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 509

• Applicable Applications for anti-DLD antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human DLD.
• SDS-PAGE MW: 54kD
• Target Name: DLD
• Preparation and Storage: Store at -20°C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using DLD antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast cancer using DLD antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human esophageal cancer using DLD antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach using DLD antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach using DLD antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse lung using DLD antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using DLD antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-DLD antibody

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Product Categories/Family for anti-DLD antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 91199540
• NCBI GeneID: 1738
• NCBI Accession #: NP_000099.2
• NCBI GenBank Nucleotide #: NM_000108.4
• UniProt Accession #: P09622; Q14131; Q14167; Q59EV8; Q8WTS4; B2R5X0; B4DHG0; B4DT69
• NCBI Official Full Name: dihydrolipoyl dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: dihydrolipoamide dehydrogenase
• NCBI Official Symbol: DLD
• NCBI Official Synonym Symbols: E3; LAD; DLDD; DLDH; GCSL; PHE3
• NCBI Protein Information: dihydrolipoyl dehydrogenase, mitochondrial
• UniProt Protein Name: Dihydrolipoyl dehydrogenase, mitochondrial
• Protein Family: Delta-like protein
• UniProt Gene Name: DLD
• UniProt Synonym Gene Names: GCSL; LAD; PHE3
• UniProt Entry Name: DLDH_HUMAN

NCBI Description

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

DLD: a multi-functional mitochondrial enzyme. An enzymatic component of the mitochondrial glycine cleavage system, the pyruvate dehydrogenase complex (PDHC), the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Is the E3 component of the PDHC that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. The E3 component has dihydrolipoamide dehydrogenase activity. The PDHC contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. Defects in DLD are a cause of maple syrup urine disease (MSUD), characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. Differentially expressed in the Wernicke's Area from patients with schizophrenia. Inhibited by 5-methoxyindole-2-carboxylic acid (MICA).

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - citrate (TCA) cycle; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase; Mitochondrial; EC 1.8.1.4; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 7q31-q32

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; acrosomal matrix; cilium

Molecular Function: mercury (II) reductase activity; FAD binding; mercury ion binding; dihydrolipoyl dehydrogenase activity; NADP binding

Biological Process: mitochondrial electron transport, NADH to ubiquinone; tricarboxylic acid cycle; gastrulation; regulation of acetyl-CoA biosynthetic process from pyruvate; branched chain family amino acid catabolic process; proteolysis; cellular metabolic process; regulation of membrane potential; cell redox homeostasis; detoxification of mercury ion; lysine catabolic process; pyruvate metabolic process; sperm capacitation

Disease: Dihydrolipoamide Dehydrogenase Deficiency