DHFR active protein

DHFR, 1-187aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203372

• Gene Names: DHFR; DYR; DHFRP1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: DHFR; 1-187aa; Human; His tag; E Coli (Bioactivity Validated); Dihydrofolate reductase; EC 1.5.1.3; DHFR active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl 2mM DTT, and 30% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND
• Sequence Length: 187

• Applicable Applications for DHFR active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 3500 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DHFR active protein

DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for DHFR active protein

Redox Proteins

References

Loveridge EJ., et al. (2009) Biochemistry. 48 (25):5922-33. ; Hu Y., et al. (2009) J Cell Biol. 185 (1):87-100.;

NCBI and Uniprot Product Information

• NCBI GI #: 4503323
• NCBI GeneID: 1719
• NCBI Accession #: NP_000782.1
• NCBI GenBank Nucleotide #: NM_000791.3
• UniProt Accession #: P00374; Q14130; Q6IRW8; B4DDD2
• Molecular Weight: 23.6 kDa (207aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: dihydrofolate reductase isoform 1
• NCBI Official Synonym Full Names: dihydrofolate reductase
• NCBI Official Symbol: DHFR
• NCBI Official Synonym Symbols: DYR; DHFRP1
• NCBI Protein Information: dihydrofolate reductase
• UniProt Protein Name: Dihydrofolate reductase
• Protein Family: Viral dihydrofolate reductase
• UniProt Gene Name: DHFR

NCBI Description

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

Uniprot Description

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.