Western Blot (WB)
(Western blot analysis of extracts of Jurkatcell line, using PPIG antibody.)
Rabbit PPIG Antibody | anti-PPIG antibody
PPIG Antibody
Gene Names
PPIG; CYP; SRCyp; SCAF10; CARS-Cyp
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
PPIG; PPIG Antibody; CARS-Cyp; CYP; MGC133241; SRCyp; CASP10; anti-PPIG antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
754
Applicable Applications for anti-PPIG antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:20-1:50
IHC: 1:50-1:100
IF: 1:20-1:50
Immunogen
Fusion protein of human PPIG
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of Jurkatcell line, using PPIG antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Jurkatcell line, using PPIG antibody.)
Related Product Information for anti-PPIG antibody
PPIG belongs to a highly conserved class of cyclophilins that function as peptidyl-prolyl-isomerases (PPIases) to catalyze the conversion of cis-proline to trans-proline in a polypeptide chain (1-4). PPIG contains an amino-terminal cyclophilin domain followed by Nopp140 repeats that are involved in its function as a nuclear chaperone (5). The carboxy-terminal of PPIG contains a SR (arginine-serine dipeptide repeat) domain (3, 4) that is involved in pre-mRNA splicing and processing (6). PPIG interacts with the carboxy-terminal domain of RNA polymerase II as well as several other SR family splicing factors. These interactions lead to changes in localization and conformation and suggest a regulatory role in transcription and pre-mRNA splicing in the elongating RNA polymerase complex (7, 8). PPIG is found in the nuclear matrix and nuclear speckles and is involved in the regulation of gene expression. PPIG shows a predominantly diffuse cytoplasmic distribution at the onset of mitosis, and in late telophase the isomerase is recruited to the newly formed nuclei (9).
Product Categories/Family for anti-PPIG antibody
References
1. Fischer, G. et al. (1989) Nature 337, 476-8. 2. Freskgård, P.O. et al. (1992) Science 258, 466-8. 3. Nestel, F.P. et al. (1996) Gene 180, 151-5. 4. Mortillaro, M.J. and Berezney, R. (1998) J Biol Chem 273, 8183-92. 5. Meier, U.T. and Blobel, G. (1992) Cell 70, 127-38. 6. Zahler, A.M. et al. (1993) Science 260, 219-22. 7. Lin, C.L. et al. (2004) Biochem Biophys Res Commun 321, 638-47. 8. Bourquin, J.P. et al. (1997) Nucleic Acids Res 25, 2055-61. 9. Dubourg, B. et al. (2004) J Biol Chem 279, 22322-30.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87kDa
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase G
NCBI Official Synonym Full Names
peptidylprolyl isomerase G
NCBI Official Symbol
PPIG
NCBI Official Synonym Symbols
CYP; SRCyp; SCAF10; CARS-Cyp
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase G
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase G
Protein Family
UniProt Gene Name
PPIG
UniProt Synonym Gene Names
PPIase G; Peptidyl-prolyl isomerase G; CARS-Cyp; CARS-cyclophilin; SR-cyclophilin; SR-cyp; SRcyp
Uniprot Description
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.
Research Articles on PPIG
Similar Products
Product Notes
The PPIG ppig (Catalog #AAA8504030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPIG Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPIG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:20-1:50. Researchers should empirically determine the suitability of the PPIG ppig for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIG, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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