SDS-Page
CNTFR active protein
CNTFR Protein, Rat, Recombinant (His Tag)
Purity
>97% as determined by SDS-PAGE
Synonyms
CNTFR; CNTFR Protein; Rat; Recombinant (His Tag); Rat CNTFR/CNTFR-alpha Protein (His Tag); ciliary neurotrophic factor receptor; CNTFR active protein
Host
Baculovirus-Insect Cells
Purity/Purification
>97% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Pro346
Species
Rat
Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat CTNFR at 10ug/ml (100uL/well) can bind biotinylated human CNTF with a linear range of 1.28-160ng/mL.
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Met 1
Tag
C-His
Protein Construction
A DNA sequence encoding the rat CNTFR (Q08406-1)(Met 1-Pro 346) was fused with a C-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for CNTFR active protein
Background: Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that have functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. Its actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracellular CNTF binding subunit(CNTFalpha) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF is also a survival factor for neurons of the peripheral sensory sympathetic, and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.
References
Dutt K, et al. (2010) Ciliary neurotrophic factor: a survival and differentiation inducer in human retinal progenitors. In Vitro Cell Dev Biol Anim. 46 (7) : 635-46.Lam A, et al. (1991) Sequence and structural organization of the human gene encoding ciliary neurotrophic factor. Gene 102 (2) : 271-6.Bazan JF. (1991) Neuropoietic cytokines in the hematopoietic fold. Neuron 7 (2) : 197-208.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,822 Da
NCBI Official Full Name
ciliary neurotrophic factor receptor subunit alpha
NCBI Official Synonym Full Names
ciliary neurotrophic factor receptor
NCBI Official Symbol
Cntfr
NCBI Protein Information
ciliary neurotrophic factor receptor subunit alpha
UniProt Protein Name
Ciliary neurotrophic factor receptor subunit alpha
Protein Family
UniProt Gene Name
Cntfr
UniProt Synonym Gene Names
CNTF receptor subunit alpha; CNTFR-alpha
NCBI Description
acts as a receptor for ciliary neurotrophic factor; may play a role in motor neuron function [RGD, Feb 2006]
Uniprot Description
Binds to CNTF. The alpha subunit provides the receptor specificity.
Research Articles on CNTFR
1. Activation of CNTF/CNTFRalpha signaling pathway by hRheb(S16H) transduction of dopaminergic neurons
Similar Products
Product Notes
The CNTFR cntfr (Catalog #AAA8123333) is an Active Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Pro34 6. It is sometimes possible for the material contained within the vial of "CNTFR, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
