Rabbit anti-Human, Mouse CDA Polyclonal Antibody | anti-CDA antibody

CDA, ID (CDA, CDD, Cytidine deaminase, Cytidine aminohydrolase) (AP)

Cat. Num. AAA6283632

• Gene Names: CDA; CDD
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CDA; Polyclonal Antibody; ID (CDA; CDD; Cytidine deaminase; Cytidine aminohydrolase) (AP); Cytidine aminohydrolase; anti-CDA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CDA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59~89 amino acids from the Center region of human CDA.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CDA Antibody (Center) in Hela cell line lysates (35ug/lane). CDA(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-CDA Antibody (Center) in mouse kidney tissue lysates (35ug/lane). CDA(arrow) was detected using the purified Pab.)

Related Product Information for anti-CDA antibody

CDA forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in the CDA gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.

Product Categories/Family for anti-CDA antibody

Antibodies; CD Markers

References

Laliberte J., Momparler R.L.Cancer Res. 54:5401-5407(1994) Demontis S., Terao M.Biochim. Biophys. Acta 1443:323-333(1998). Kuhn K., Bertling W.M., Emmrich F.Biochem. Biophys. Res. Commun. 190:1-7(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 1705718
• NCBI GeneID: 978
• UniProt Accession #: P32320
• Molecular Weight: 146
• NCBI Official Full Name: Cytidine deaminase
• NCBI Official Synonym Full Names: cytidine deaminase
• NCBI Official Symbol: CDA
• NCBI Official Synonym Symbols: CDD
• NCBI Protein Information: cytidine deaminase; cytidine aminohydrolase; small cytidine deaminase; cytosine nucleoside deaminase
• UniProt Protein Name: Cytidine deaminase
• Protein Family: Colicin
• UniProt Gene Name: CDA
• UniProt Synonym Gene Names: CDD
• UniProt Entry Name: CDD_HUMAN

NCBI Description

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]

Uniprot Description

CDA: This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: Nucleotide Metabolism - pyrimidine; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.5.4.5

Chromosomal Location of Human Ortholog: 1p36.2-p35

Cellular Component: extracellular region; cytosol

Molecular Function: protein homodimerization activity; zinc ion binding; nucleoside binding; cytidine deaminase activity

Biological Process: cell surface receptor linked signal transduction; pyrimidine base metabolic process; cytosine metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; cytidine deamination; negative regulation of cell growth; pyrimidine salvage; negative regulation of nucleotide metabolic process; protein homotetramerization