Carbonic anhydrase 2 Active Protein | CA2 active protein

Carbonic anhydrase 2, 1-260aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA203326

• Gene Names: CA2; CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Carbonic anhydrase 2; 1-260aa; Human; E Coli (Bioactivity Validated); CA-II; CAC; CA 2; CA II; CA2; CAI; Car 2; Car2; Carbonate dehydratase II; Carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; Carbonic dehydratase.; CA2 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 50mM NaCl, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK
• Sequence Length: 260

• Applicable Applications for CA2 active protein: SDS-PAGE
• Antigen Species: Human
• Biological Activity: Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CA2 active protein

Carbonic anhydrase 2 (CA2) is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. Recombinant human CA2 was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for CA2 active protein

Enzymes & Proteases

References

Hu PY., et al. (1992). Hum Mutat. 1 (4):288-92 ; Venta PJ., et al. (1991). Am J Hum Genet. 49 (5):1082-90;

NCBI and Uniprot Product Information

• NCBI GI #: 4557395
• NCBI GeneID: 760
• NCBI Accession #: NP_000058
• NCBI GenBank Nucleotide #: NM_000067.2
• UniProt Accession #: P00918; Q6FI12; Q96ET9; B2R7G8
• Molecular Weight: 29.2 kDa (260aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: carbonic anhydrase 2 isoform 1
• NCBI Official Synonym Full Names: carbonic anhydrase 2
• NCBI Official Symbol: CA2
• NCBI Official Synonym Symbols: CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282
• NCBI Protein Information: carbonic anhydrase 2
• UniProt Protein Name: Carbonic anhydrase 2
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA2
• UniProt Synonym Gene Names: CAC; CA-II

NCBI Description

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

Essential for bone resorption and osteoclast differentiation (). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.