Carbonic anhydrase 1 Active Protein | CA1 active protein

Carbonic anhydrase 1, 1-261 aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA206225

• Gene Names: CA1; CAB; CA-I; Car1; HEL-S-11
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Carbonic anhydrase 1; 1-261 aa; Human; His tag; E Coli (Bioactivity Validated); CA1; CA-I; CAB; CA1 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
• Sequence Length: 261

• Applicable Applications for CA1 active protein: Enzyme Activity; SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CA1 active protein

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O <-> HCO3- + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E Coli and purified by conventional chromatography techniques.

Product Categories/Family for CA1 active protein

Metabolism; Differentiation & Regeneration

References

Nogradi A., et al. (1998). Am J Pathol. 153:1-4.; Ferry JG., et al. (1999). Proc Natl Acad Sci U S A. 96 (26):15184-9.

NCBI and Uniprot Product Information

• NCBI GI #: 192447430
• NCBI GeneID: 759
• NCBI Accession #: NP_001122301
• NCBI GenBank Nucleotide #: NM_001128829.3
• UniProt Accession #: P00915
• Molecular Weight: 31.0 kDa (281aa), confirmed by MALDI-TOF
• NCBI Official Full Name: carbonic anhydrase 1 isoform a
• NCBI Official Synonym Full Names: carbonic anhydrase 1
• NCBI Official Symbol: CA1
• NCBI Official Synonym Symbols: CAB; CA-I; Car1; HEL-S-11
• NCBI Protein Information: carbonic anhydrase 1
• UniProt Protein Name: Carbonic anhydrase 1
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA1
• UniProt Synonym Gene Names: CAB; CA-I

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Nov 2016]

Uniprot Description

Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.