CA199 (FUT3) Protein Active Protein | CA199 active protein

Recombinant Human CA199 (FUT3) Protein (His Tag)

Cat. Num. AAA2569258

• Gene Names: FUT3; LE; Les; FT3B; CD174; FucT-III
• Purity: >80% as determined by reducing SDS-PAGE
• Synonyms: CA199 (FUT3) Protein; Recombinant Human CA199 (FUT3) Protein (His Tag); Galactoside 3(4)-L-fucosyltransferase; Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III; FucT-III; FUT3; FT3B; LE; CA199 active protein

• Host: E Coli
• Purity/Purification: >80% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4
• Sequence: Val35-Thr361

• Species: Human
• Tag: N-His
• Protein Construction: A DNA sequence encoding the Human FUT3 (P21217-1)(Val35-Thr361) was expressed with a polyhistidine tag at the N-terminus.
• Activity: Immunogen (E-AB-40309)
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for CA199 active protein

May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.

NCBI and Uniprot Product Information

• NCBI GI #: 4503809
• NCBI GeneID: 2525
• NCBI Accession #: NP_000140.1
• NCBI GenBank Nucleotide #: NM_000149.3
• UniProt Accession #: P21217; Q32NE7; Q99448; Q99449; B5U7U9; B5U7V0
• Molecular Weight: 42,117 Da
• NCBI Official Full Name: galactoside 3(4)-L-fucosyltransferase
• NCBI Official Synonym Full Names: fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
• NCBI Official Symbol: FUT3
• NCBI Official Synonym Symbols: LE; Les; FT3B; CD174; FucT-III
• NCBI Protein Information: galactoside 3(4)-L-fucosyltransferase; galactoside 3(4)-L-fucosyltransferase; Lewis FT; fucosyltransferase III; alpha-(1,3/1,4)-fucosyltransferase; blood group Lewis alpha-4-fucosyltransferase
• UniProt Protein Name: Galactoside 3(4)-L-fucosyltransferase
• UniProt Gene Name: FUT3
• UniProt Synonym Gene Names: FT3B; LE; Lewis FT; FucT-III
• UniProt Entry Name: FUT3_HUMAN

NCBI Description

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FUT3: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. Belongs to the glycosyltransferase 10 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase; Membrane protein, integral; EC 2.4.1.65

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; integral to membrane

Molecular Function: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity

Biological Process: cell-cell recognition; biopolymer glycosylation; oligosaccharide biosynthetic process; protein amino acid glycosylation