Arginine Deiminase (ADI) Active Protein | ADI active protein
Mycoplasma Arginine Deiminase (ADI)
Purity
> 98% by SDS-PAGE & HPLC analysis
Synonyms
Arginine Deiminase (ADI); Mycoplasma Arginine Deiminase (ADI); ADI; ADI active protein
Host
E Coli
Purity/Purification
> 98% by SDS-PAGE & HPLC analysis
Form/Format
Lyophilized from 2mM Sodium Phosphate, pH7.5 + 0.2mM DTT
Sequence
SVFDSKFKGI HVYSEIGELE SVLVHEPGRE IDYITPARLD ELLFSA ILES HDARKEHKQF VAELKANDIN VVELIDLVAE TYDLASQEAK D KLIEEFLED SEPVLSEEHK VVVRNFLKAK KTSRELVEIM MAGITKT DLG IEADHELIVD PMPNLYFTRD PFASVGNGVT IHYMRYKVRQ RE TLFSRFVF SNHPKLINTP WYYDPSLKLS IEGGDVFIYN NDTLVVGV SE RTDLQTVTLL AKNIVANKEC EFKRIVAINV PKWTNLMHLD TWL TMLDKDK FLYSPIANDV FKFWDYDLVN GGAEPQPVEN GLPLEGLLQ S IINKKPVLIP IAGEGASQME IERETHFDGT NYLAIRPGVV IGYS RNEKTN AALEAAGIKV LPFHGNQLSL GMGNARCMSM PLSRKDVKW
Sequence Length
409
Endotoxin level
< 0.1 ng /ug of protein (<1EU/ug).
Biological Activity
Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.
Protein RefSeq
CAA38080.1
mRNA RefSeq
AF182646.1
Reconstitution
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
Preparation and Storage
The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 3 months. Reconstituted working aliquots are stable for 1 week at 2 degree C to 8 degree C and for 3 months at -20 degree C to -80 degree C.
Related Product Information for ADI active protein
Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids.
Product Categories/Family for ADI active protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
46.3kDa
NCBI Official Full Name
Arginine deiminase
UniProt Protein Name
Arginine deiminase
Protein Family
UniProt Gene Name
arcA
UniProt Synonym Gene Names
ADI; AD
UniProt Entry Name
ARCA_MYCAR
Uniprot Description
Catalytic activity: L-arginine + H2O = L-citrulline + NH3. HAMAP-Rule MF_00242
Pathway: Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 1/2. HAMAP-Rule MF_00242
Subunit structure: Homodimer.
Subcellular location: Cytoplasm
Potential HAMAP-Rule MF_00242.
Sequence similarities: Belongs to the arginine deiminase family.
Similar Products
Product Notes
The ADI arca (Catalog #AAA691980) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: SVFDSKFKGI HVYSEIGELE SVLVHEPGRE IDYITPARLD ELLFSA ILES HDARKEHKQF VAELKANDIN VVELIDLVAE TYDLASQEAK D KLIEEFLED SEPVLSEEHK VVVRNFLKAK KTSRELVEIM MAGITKT DLG IEADHELIVD PMPNLYFTRD PFASVGNGVT IHYMRYKVRQ RE TLFSRFVF SNHPKLINTP WYYDPSLKLS IEGGDVFIYN NDTLVVGV SE RTDLQTVTLL AKNIVANKEC EFKRIVAINV PKWTNLMHLD TWL TMLDKDK FLYSPIANDV FKFWDYDLVN GGAEPQPVEN GLPLEGLLQ S IINKKPVLIP IAGEGASQME IERETHFDGT NYLAIRPGVV IGYS RNEKTN AALEAAGIKV LPFHGNQLSL GMGNARCMSM PLSRKDVKW. It is sometimes possible for the material contained within the vial of "Arginine Deiminase (ADI), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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