AKR1C4 active protein

Human Recombinant AKR1C4

Cat. Num. AAA844237

• Gene Names: AKR1C4; C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD
• Applications: SDS-Page
• Purity: >=90% by SDS-PAGE
• Synonyms: AKR1C4; Human Recombinant AKR1C4; Aldo-keto reductase family 1; member C 4; DHEA-ST; DHEAS; HST; hSTa; ST2; ST2A1; ST2A3.; AKR1C4 active protein

• Host: E Coli
• Purity/Purification: >=90% by SDS-PAGE
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl and 1 mM DTT.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY
• Sequence Length: 323

• Applicable Applications for AKR1C4 active protein: SDS-PAGE
• Biological Activity: Specific activity is approximately < 0.6 units/mg.
• Unit Definition: Specific activity is approximately < 0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25 degree C. Specific activity was expressed as units/mg protein.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Testing Data

Related Product Information for AKR1C4 active protein

Background: AKR1C4 is a member of the aldo/keto reductase superfamily that has over 40 known enzymes and proteins. AKR1C4 enables the conversion of aldehydes and ketones to their corresponding alcohols by using NADH and/or NADPH as cofactors. AKR1C4 takes part in the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver.

Product Categories/Family for AKR1C4 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  

NCBI and Uniprot Product Information

• NCBI GI #: 325652083
• NCBI GeneID: 1109
• NCBI Accession #: NP_001809.3
• NCBI GenBank Nucleotide #: NM_001818.3
• UniProt Accession #: P17516; Q5T6A3; Q8WW84; Q9NS54; AAB47003
• Molecular Weight: 39.2 kDa (343 aa, 1-323 aa + His Tag (NT))
• NCBI Official Full Name: aldo-keto reductase family 1 member C4
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C4
• NCBI Official Symbol: AKR1C4
• NCBI Official Synonym Symbols: C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD
• NCBI Protein Information: aldo-keto reductase family 1 member C4
• UniProt Protein Name: Aldo-keto reductase family 1 member C4
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C4
• UniProt Synonym Gene Names: CHDR; CDR; DD-4; DD4
• UniProt Entry Name: AK1C4_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

Uniprot Description

AKR1C4: Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha- androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20- alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. Defects in AKR1C4 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. AKR1C4 mutations may act as modifier of disease severity in SRXY8 patients. A splicing mutation resulting in loss of exon 2 has been found in affected individuals also carrying mutation Val-79 in AKR1C2 (PubMed:21802064). Belongs to the aldo/keto reductase family.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.357; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - primary bile acid biosynthesis; Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.1.1.225

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm; cytosol

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; electron carrier activity; aldo-keto reductase activity; chlordecone reductase activity; retinal dehydrogenase activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; bile acid transmembrane transporter activity

Biological Process: steroid metabolic process; bile acid and bile salt transport; phototransduction, visible light; bile acid biosynthetic process; bile acid metabolic process; androgen metabolic process; retinoid metabolic process

Disease: 46,xy Sex Reversal 8