DYRK3 / REDK recombinant protein

Recombinant Human DYRK3 / REDK Protein (His & GST tag)

Cat. Num. AAA2545092

• Gene Names: DYRK3; RED; REDK; DYRK5; hYAK3-2
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: DYRK3 / REDK; Recombinant Human DYRK3 / REDK Protein (His & GST tag); RP11-343H5.2; DYRK5; RED; REDK; hYAK3-2; DYRK3 / REDK recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, 10% gly, pH 8.0
• Sequence Length: 568

• Application Notes: The recombinant human DYRK3/GST chimera consists of 825 amino acids and has a calculated molecular mass of 93.5 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for DYRK3 / REDK recombinant protein

Background: Dual specificity tyrosine-phosphorylation-regulated kinase 3, also known as Regulatory erythroid kinase, REDK and DYRK3, is a nucleus protein which belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MNB/DYRK subfamily. DYRKs are an emerging family of dual-specificity kinases that play key roles in cell proliferation, survival, and development. DYRK3 contains one protein kinase domain. Isoform 1 and isoform 2 of DYRK3 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 of DYRK3 is the predominant form in testis. Isoform 1 of DYRK3 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus. DYRK3 is a negative regulator of EPO-dependent erythropoiesis. It may place an upper limit on red cell production during stress erythropoiesis. DYRK3 inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. It may also act by regulating CREB/CRE signaling. DYRK3 proved to effectively inhibit NFAT (nuclear factor of activated T cells) transcriptional response pathways and to co-immunoprecipitate with NFATc3. DYRK3 attenuates (and possibly apportions) red cell production selectively during anemia.

Description: A DNA sequence encoding the human DYRK3 isoform 1 (O43781-1) (Met 1-Ser 588) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 51702242
• NCBI GeneID: 8444
• NCBI Accession #: NP_001004023.1
• NCBI GenBank Nucleotide #: NM_001004023.1
• UniProt Accession #: O43781; Q7Z752; Q9HBY6; Q9HBY7; D3DT79
• Molecular Weight: 63,977 Da
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 3 isoform b
• NCBI Official Synonym Full Names: dual specificity tyrosine phosphorylation regulated kinase 3
• NCBI Official Symbol: DYRK3
• NCBI Official Synonym Symbols: RED; REDK; DYRK5; hYAK3-2
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 3
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 3
• UniProt Gene Name: DYRK3
• UniProt Synonym Gene Names: REDK

NCBI Description

This gene product belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. The members of this family share structural similarity, however, differ in their substrate specificity, suggesting their involvement in different cellular functions. The encoded protein has been shown to autophosphorylate on tyrosine residue and catalyze phosphorylation of histones H3 and H2B in vitro. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

DYRK3: a dual-specificity protein kinase of the DYRK family. Localizes in the cytoplasm. Expressed primarily in erythroid progenitor cells and regulates late erythropoiesis.

Protein type: CMGC group; DYRK family; Dyrk2 subfamily; EC 2.7.12.1; Kinase, protein; Protein kinase, CMGC; Protein kinase, dual-specificity (non-receptor)

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleus

Molecular Function: ATP binding; magnesium ion binding; protein kinase activity

Biological Process: erythrocyte differentiation; protein amino acid phosphorylation