Pig Dual oxidase 2 ELISA Kit | DUOX2 elisa kit

Pig Dual oxidase 2 (DUOX2) ELISA Kit

Cat. Num. AAA282739

• Reactivity: Pig
• Synonyms: Dual oxidase 2; Pig Dual oxidase 2 (DUOX2) ELISA Kit; DUOX2 elisa kit

• Reactivity: Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pig DUOX2. No significant cross-reactivity or interference between Pig DUOX2 and analogues was observed.
• Sequence Length: 1545

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DUOX2 elisa kit

Dual oxidase is an enzyme that was first identified in the mammalian thyroid gland. In humans, two isoforms are found; hDUOX1 and hDUOX2 (this enzyme). The protein location is not exclusive to thyroid tissue; hDUOX1 is prominent in airway epithelial cells and hDUOX2 in the salivary glands and gastrointestinal tract.It has been suggested that DUOX2 is the isoform to generate H2O2 utilized by thyroid peroxidase (TPO) for the biosynthesis of thyroid hormones, supported by the discovery of congenital hypothyroidism resultant from an inactivating mutation in the DUOX2 gene. The family currently has seven members including NOX1, NOX2 (formerly known as gp91phox), NOX3, NOX4, NOX5, DUOX1 and DUOX2.This protein is known as a dual oxidase because it has both a peroxidase homology domain and a gp91phox domain.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DUOX2 in samples. An antibody specific for DUOX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDUOX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUOX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUOX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 51889718
• NCBI GeneID: 397060
• NCBI Accession #: NP_999164.2
• NCBI GenBank Nucleotide #: NM_213999.2
• UniProt Accession #: Q8HZK2; Q9TT98
• Molecular Weight: 175,284 Da
• NCBI Official Full Name: dual oxidase 2
• NCBI Official Symbol: DUOX2
• NCBI Protein Information: dual oxidase 2
• UniProt Protein Name: Dual oxidase 2
• Protein Family: Dual oxidase
• UniProt Gene Name: DUOX2

Uniprot Description

Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.