Rabbit anti-Human, Mouse FBLN1 Polyclonal Antibody | anti-FBLN1 antibody

FBLN1, CT (FBLN1, Fibulin-1) (FITC)

Cat. Num. AAA6298156

• Gene Names: FBLN1; FBLN; FIBL1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBLN1; Polyclonal Antibody; CT (FBLN1; Fibulin-1) (FITC); Fibulin-1; anti-FBLN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FBLN1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FBLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 621-650 amino acids from the C-terminal region of human FBLN1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBLN1 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the FBLN1 antibody detected the FBLN1 protein (arrow).)

Western Blot (WB)

(FBLN1 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the FBLN1 antibody detected the FBLN1 protein (arrow).)

Immunohistochemistry (IHC)

(FBLN1 Antibody (C-term) (AP14671b)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FBLN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-FBLN1 antibody

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants.

Product Categories/Family for anti-FBLN1 antibody

Antibodies; Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Li, C., et al. Mol. Vis. 16, 689-697 (2010): Wooten, E.C., et al. PLoS ONE 5 (1), E8830 (2010): Argraves, W.S., et al. Histochem. Cell Biol. 132(5):559-565(2009). Piscaglia, F., et al. Cell Tissue Res. 337(3):449-462(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 34734062
• NCBI GeneID: 2192
• NCBI Accession #: NP_001987.2; NP_006477.2
• NCBI GenBank Nucleotide #: NM_001996.3
• UniProt Accession #: P23142; P23143; P23144; P37888; Q5TIC4; Q8TBH8; Q9HBQ5; Q9UC21; Q9UGR4; Q9UH41; B0QY42; B1AHL4
• Molecular Weight: 74,434 Da
• NCBI Official Full Name: fibulin-1 isoform C
• NCBI Official Synonym Full Names: fibulin 1
• NCBI Official Symbol: FBLN1
• NCBI Official Synonym Symbols: FBLN; FIBL1
• NCBI Protein Information: fibulin-1
• UniProt Protein Name: Fibulin-1
• UniProt Gene Name: FBLN1
• UniProt Synonym Gene Names: FIBL-1
• UniProt Entry Name: FBLN1_HUMAN

NCBI Description

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008]

Uniprot Description

FBLN1: Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP. A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation. Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane

Molecular Function: protease activator activity; extracellular matrix structural constituent; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; viral reproduction; embryo implantation

Disease: Synpolydactyly 2