Rabbit LIG4 Polyclonal Antibody | anti-LIG4 antibody

LIG4 (DNA Ligase 4) (FITC)

Cat. Num. AAA6424739

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by affinity chromatography.
• Synonyms: LIG4; Polyclonal Antibody; LIG4 (DNA Ligase 4) (FITC); anti-LIG4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LIG4. Species Crossreactivity: mouse and rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-LIG4 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant fusion protein corresponding to amino acids 612-911 of human LIG4.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using 211762 at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-LIG4 antibody

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

Product Categories/Family for anti-LIG4 antibody

Antibodies; DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 148539894
• NCBI GeneID: 3981
• NCBI Accession #: NP_001091738.1
• NCBI GenBank Nucleotide #: NM_001098268.1
• UniProt Accession #: P49917; Q8IY66; Q8TEU5
• Molecular Weight: 103,971 Da
• NCBI Official Full Name: DNA ligase 4
• NCBI Official Synonym Full Names: ligase IV, DNA, ATP-dependent
• NCBI Official Symbol: LIG4
• NCBI Protein Information: DNA ligase 4; sealase; DNA joinase; DNA ligase IV; DNA repair enzyme; polynucleotide ligase; polydeoxyribonucleotide synthase [ATP] 4
• UniProt Protein Name: DNA ligase 4
• Protein Family: DNA ligase
• UniProt Gene Name: LIG4
• UniProt Entry Name: DNLI4_HUMAN

NCBI Description

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

LIG4: a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. Defects in this gene are the cause of LIG4 syndrome.

Protein type: EC 6.5.1.1; Ligase

Chromosomal Location of Human Ortholog: 13q33-q34

Cellular Component: nucleoplasm; DNA ligase IV complex; focal adhesion; cytoplasm; plasma membrane; condensed chromosome; DNA-dependent protein kinase complex; nucleus

Molecular Function: protein C-terminus binding; protein binding; DNA binding; DNA ligase activity; metal ion binding; DNA ligase (ATP) activity; ATP binding; ligase activity

Biological Process: central nervous system development; viral reproduction; somatic stem cell maintenance; T cell differentiation in the thymus; chromosome organization and biogenesis; double-strand break repair via nonhomologous end joining; DNA ligation; positive regulation of fibroblast proliferation; double-strand break repair; DNA ligation during DNA repair; response to gamma radiation; negative regulation of neuron apoptosis; DNA ligation during DNA recombination; response to X-ray; pro-B cell differentiation; single strand break repair; positive regulation of neurogenesis; V(D)J recombination; immunoglobulin V(D)J recombination; in utero embryonic development; isotype switching; cell cycle; DNA repair; lagging strand elongation; cell proliferation; neuron apoptosis; cell division; T cell receptor V(D)J recombination; nucleotide-excision repair, DNA gap filling

Disease: Myeloma, Multiple; Lig4 Syndrome