Disulfide Oxidoreductase Recombinant Protein | dsbI recombinant protein
Disulfide Oxidoreductase, Recombinant (dsbA)
Purity
Highly Purified95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Synonyms
Disulfide Oxidoreductase; Recombinant (dsbA); dsbI recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form/Format
Supplied as a lyophilized powder in 50mM sodium phosphate, 100mM sodium chloride.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ly-Lys-Ala-Trp.
Application Notes
1. Western Blot (we recommend that the material be diluted in 1X SDS-PAGE sample buffer (1). On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a colorimetric Western Blot at a dilution of 1:10,000 as the primary antibody and an appropriate alkaline phosphatase conjugated secondary antibody for detection.)
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of dsbA.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of dsbA as a Reference Standard.
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of dsbA as a Reference Standard.
Reconstitution
Reconstitute the lyophilized Human dsbA in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for dsbI recombinant protein
DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli.
DsbA is a periplasmic protein isolated from E. coli having a molecular mass of 23,149 Dalton.
DsbA is a periplasmic protein isolated from E. coli having a molecular mass of 23,149 Dalton.
Product Categories/Family for dsbI recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,399 Da
NCBI Official Full Name
disulfide oxidoreductase
UniProt Protein Name
Putative protein-disulfide oxidoreductase DsbI
Protein Family
UniProt Gene Name
dsbI
UniProt Entry Name
DSBI_CAMJE
Uniprot Description
Function: Required for disulfide bond formation in some proteins. Part of a redox system composed of DsbI and DsbL that mediates formation of an essential disulfide bond in AssT
By similarity.
Subunit structure: Interacts with DsbL
By similarity.
Subcellular location: Cell inner membrane; Multi-pass membrane protein
By similarity.
Sequence similarities: Belongs to the DsbB family. DsbI subfamily.
Caution: Was originally given the gene name dsbB; however this seems to belong to a different DsbB subfamily.
Similar Products
Product Notes
The Disulfide Oxidoreductase dsbi (Catalog #AAA636975) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. 1. Western Blot (we recommend that the material be diluted in 1X SDS-PAGE sample buffer (1). On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a colorimetric Western Blot at a dilution of 1:10,000 as the primary antibody and an appropriate alkaline phosphatase conjugated secondary antibody for detection.). Researchers should empirically determine the suitability of the Disulfide Oxidoreductase dsbi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ly-Lys -Ala-Trp. It is sometimes possible for the material contained within the vial of "Disulfide Oxidoreductase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
