Human Dihydropyrimidine Dehydrogenase ELISA Kit | DPYD elisa kit

Human Dihydropyrimidine Dehydrogenase ELISA Kit

Cat. Num. AAA761075

• Gene Names: DPYD; DHP; DPD; DHPDHASE
• Reactivity: Human
• Synonyms: Dihydropyrimidine Dehydrogenase; Human Dihydropyrimidine Dehydrogenase ELISA Kit; DPYD/Dihydropyrimidine Dehydrogenase; DPYD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Agt. No significant cross-reactivity or interference between Agt and analogues was observed.
• Sequence Length: 1025

• Samples: Cell, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPYD elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 693912
• NCBI GeneID: 1806
• Molecular Weight: 18,936 Da
• NCBI Official Full Name: dihydropyrimidine dehydrogenase
• NCBI Official Synonym Full Names: dihydropyrimidine dehydrogenase
• NCBI Official Symbol: DPYD
• NCBI Official Synonym Symbols: DHP; DPD; DHPDHASE
• NCBI Protein Information: dihydropyrimidine dehydrogenase [NADP(+)]
• UniProt Protein Name: Dihydropyrimidine dehydrogenase [NADP(+)]
• Protein Family: Dihydropyrimidine dehydrogenase
• UniProt Gene Name: DPYD
• UniProt Synonym Gene Names: DHPDHase; DPD
• UniProt Entry Name: DPYD_HUMAN

NCBI Description

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: EC 1.3.1.2; Other Amino Acids Metabolism - beta-alanine; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytoplasm; cytosol

Molecular Function: 4 iron, 4 sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; FAD binding; metal ion binding; NADP binding; protein binding; protein homodimerization activity

Biological Process: beta-alanine biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; purine base catabolic process; pyrimidine base catabolic process; pyrimidine base metabolic process; pyrimidine nucleoside catabolic process; thymidine catabolic process; thymine catabolic process; uracil catabolic process

Disease: Dihydropyrimidine Dehydrogenase Deficiency