Human Glucokinase ELISA Kit | GCK elisa kit

Human Glucokinase ELISA Kit

Cat. Num. AAA763461

• Gene Names: gck; glk; hk4; hhf3; hkiv; hxkp; mody2; glucokinase
• Reactivity: Human
• Synonyms: Glucokinase; Human Glucokinase ELISA Kit; GCK (Glucokinase)/FGQTL3/GLK/Hexokinase-4/Hexokinase-D/HHF3/HK4/HXKP/LGLK/GK/glucokinase (hexokinase 4)/HKIV/maturity onset diabetes of the young 2/MODY2; GCK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GCA. No significant cross-reactivity or interference between GCA and analogues was observed.
• Sequence Length: 458

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GCA were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GCA were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GCK elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GCA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GCA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GCA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GCA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 156717562
• NCBI GeneID: 100124905
• NCBI Accession #: NP_001096321.1
• NCBI GenBank Nucleotide #: NM_001102851.1
• NCBI Official Full Name: glucokinase
• NCBI Official Synonym Full Names: glucokinase (hexokinase 4)
• NCBI Official Symbol: gck
• NCBI Official Synonym Symbols: glk; hk4; hhf3; hkiv; hxkp; mody2; glucokinase
• NCBI Protein Information: glucokinase
• Protein Family: Glucokinase