Typical Testing Data/Standard Curve (for reference only)
(Human Granulysin PicoKine ELISA Kit standard curve )
Human Granulysin ELISA Kit | GNLY elisa kit
Human Granulysin PicoKine ELISA Kit
Gene Names
GNLY; 519; LAG2; NKG5; LAG-2; D2S69E; TLA519
Reactivity
Human
Synonyms
Granulysin; Human Granulysin PicoKine ELISA Kit; granulysin; 519; D2S69E; GNLY; GNLY_HUMAN; LAG 2; LAG2; Lymphocyte activation gene 2; Lymphokine LAG 2; Lymphokine LAG-2; NKG 5; NKG5; Protein NKG5; T cell activation protein 519; T lymphocyte activation gene 519; T-cell activation protein 519; TLA519; GNLY elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Granulysin
Sequence Length
145
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human Granulysin PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human Granulysin PicoKine ELISA Kit standard curve )
Related Product Information for GNLY elisa kit
Principle of the assay: human Granulysin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Granulysin has been precoated onto 96-well plates. Standards(NSO, M1-L145) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Granulysin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Granulysin amount of sample captured in plate.
Background: Granulysin is a substance released by cytotoxic T cells(CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein(SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.
Background: Granulysin is a substance released by cytotoxic T cells(CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein(SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,853 Da
NCBI Official Full Name
Granulysin
NCBI Official Synonym Full Names
granulysin
NCBI Official Symbol
GNLY
NCBI Official Synonym Symbols
519; LAG2; NKG5; LAG-2; D2S69E; TLA519
NCBI Protein Information
granulysin; lymphokine LAG-2; lymphocyte-activation gene 2; T-cell activation protein 519; T-lymphocyte activation gene 519
UniProt Protein Name
Granulysin
Protein Family
UniProt Gene Name
GNLY
UniProt Synonym Gene Names
LAG2; NKG5; TLA519
UniProt Entry Name
GNLY_HUMAN
NCBI Description
The product of this gene is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. This protein is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
GNLY: Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: extracellular space
Biological Process: killing of cells of another organism; defense response to bacterium; cellular defense response; defense response to fungus
Research Articles on GNLY
Similar Products
Product Notes
The Human GNLY gnly (Catalog #AAA177194) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177194 ELISA Kit recognizes Human GNLY. It is sometimes possible for the material contained within the vial of "Granulysin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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