Rat Deoxyhypusine hydroxylase ELISA Kit | DOHH elisa kit
Rat Deoxyhypusine hydroxylase (DOHH) ELISA Kit
Gene Names
Dohh; RGD1304783
Reactivity
Rat
Synonyms
Deoxyhypusine hydroxylase; Rat Deoxyhypusine hydroxylase (DOHH) ELISA Kit; HLRC1; MGC4293; HEAT-like (PBS lyase) repeat containing 1|deoxyhypusine dioxygenase; DOHH elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat DOHH. No significant cross-reactivity or interference between Rat DOHH and analogues was observed.
Sequence Length
302
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for DOHH elisa kit
DOHH encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.
DOHH contains 8 heat repeats organized in a symmetrical dyad connected by a variable region. It contains 2 potential metal coordination sites, each composed of 2 strictly conserved his-glu motifs.CD spectroscopy of purified recombinant wildtype DOHH indicated an alpha-helix-rich protein,and site-directed mutagenesis showed that the alpha-helical structure of DOHH does not depend on the conserved his-glu motifs or metal binding.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DOHH in samples. An antibody specific for DOHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDOHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DOHH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DOHH bound in the initial step. The color development is stopped and the intensity of the color is measured.
DOHH contains 8 heat repeats organized in a symmetrical dyad connected by a variable region. It contains 2 potential metal coordination sites, each composed of 2 strictly conserved his-glu motifs.CD spectroscopy of purified recombinant wildtype DOHH indicated an alpha-helix-rich protein,and site-directed mutagenesis showed that the alpha-helical structure of DOHH does not depend on the conserved his-glu motifs or metal binding.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DOHH in samples. An antibody specific for DOHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDOHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DOHH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DOHH bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,077 Da
NCBI Official Full Name
deoxyhypusine hydroxylase
NCBI Official Synonym Full Names
deoxyhypusine hydroxylase
NCBI Official Symbol
Dohh
NCBI Official Synonym Symbols
RGD1304783
NCBI Protein Information
deoxyhypusine hydroxylase
UniProt Protein Name
Deoxyhypusine hydroxylase
Protein Family
UniProt Gene Name
Dohh
UniProt Synonym Gene Names
DOHH
Uniprot Description
Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
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Product Notes
The Rat DOHH dohh (Catalog #AAA285898) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285898 ELISA Kit recognizes Rat DOHH. It is sometimes possible for the material contained within the vial of "Deoxyhypusine hydroxylase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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