Western Blot (WB)
(Staining (0. 03ug/ml) of human HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat GIPC1/NIP Polyclonal Antibody | anti-GIPC1 antibody
Goat anti-GIPC1/NIP Antibody
Gene Names
GIPC1; NIP; GIPC; IIP-1; TIP-2; SEMCAP; C19orf3; Hs.6454; GLUT1CBP; RGS19IP1; SYNECTIN; SYNECTIIN
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GIPC1/NIP; Polyclonal Antibody; Goat anti-GIPC1/NIP Antibody; GIPC1; NIP; GIPC PDZ domain containing family; member 1; C19orf3; GIPC; GLUT1CBP; Hs. 6454; IIP-1; MGC15889; MGC3774; RGS19IP1; SEMCAP; SYNECTIIN; TIP-2; GLUT1 C-terminal binding protein; IGF-1 receptor interacting protein 1; regulator of G-protein signall; GIPC1 antibody; NIP antibody; member 1 antibody; C19orf3 antibody; GIPC antibody; GLUT1CBP antibody; Hs. 6454 antibody; IIP-1 antibody; MGC15889 antibody; MGC3774 antibody; RGS19IP1 antibody; SEMCAP antibody; SYNECTIIN antibody; TIP-2 antibody; GLUT1 C-terminal binding protein antibody; IGF-1 receptor interacting protein 1 antibody; regulator of G-protein signalling 19 interacting protein 1 antibody; synectin antibody; SYNECTIN antibody; GIPC1 / NIP; anti-GIPC1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
GAIGDAKVGRY
Sequence Length
333
Applicable Applications for anti-GIPC1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, and approx 24kDa also observed in Human Pancreas, Kidney and Muscle lysates (calculated MW of 36. 0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 03-0.1ug/ml.
Western Blot: Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, and approx 24kDa also observed in Human Pancreas, Kidney and Muscle lysates (calculated MW of 36. 0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 03-0.1ug/ml.
Immunogen
Peptide with sequence C-GAIGDAKVGRY, from the C Terminus of the protein sequence according to NP_005707.1; NP_974197.1; NP_974199.1; NP_974196.1; NP_974198.1; NP_974223.1.
Epitope
C Terminus
Note
This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Staining (0. 03ug/ml) of human HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0. 03ug/ml) of human HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing GIPC1 isoform 1 (RC216466) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing GIPC1 isoform 1 (RC216466) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,072 Da
NCBI Official Full Name
PDZ domain-containing protein GIPC1 isoform 1
NCBI Official Synonym Full Names
GIPC PDZ domain containing family member 1
NCBI Official Symbol
GIPC1
NCBI Official Synonym Symbols
NIP; GIPC; IIP-1; TIP-2; SEMCAP; C19orf3; Hs.6454; GLUT1CBP; RGS19IP1; SYNECTIN; SYNECTIIN
NCBI Protein Information
PDZ domain-containing protein GIPC1
UniProt Protein Name
PDZ domain-containing protein GIPC1
Protein Family
UniProt Gene Name
GIPC1
UniProt Synonym Gene Names
C19orf3; GIPC; RGS19IP1; TIP-2
UniProt Entry Name
GIPC1_HUMAN
NCBI Description
GIPC1 is a scaffolding protein that regulates cell surface receptor expression and trafficking (Lee et al., 2008 [PubMed 18775991]).[supplied by OMIM, Apr 2009]
Uniprot Description
GIPC1: May be involved in G protein-linked signaling. Belongs to the GIPC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; GAPs; GAPs, misc.; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19p13.1
Cellular Component: brush border; cell cortex; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; dendritic shaft; dendritic spine; endocytic vesicle; membrane; synaptic vesicle; vesicle membrane
Molecular Function: actin binding; myosin binding; PDZ domain binding; protein binding; protein homodimerization activity; receptor binding
Biological Process: endothelial cell migration; G-protein coupled receptor protein signaling pathway; glutamate secretion; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cytokinesis; positive regulation of transforming growth factor beta receptor signaling pathway; protein targeting; regulation of protein stability; regulation of synaptic plasticity; synaptic transmission
Research Articles on GIPC1
Similar Products
Product Notes
The GIPC1 gipc1 (Catalog #AAA421016) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GIPC1/NIP Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's GIPC1/NIP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, and approx 24kDa also observed in Human Pancreas, Kidney and Muscle lysates (calculated MW of 36. 0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the GIPC1 gipc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GAIGDAKVGR Y. It is sometimes possible for the material contained within the vial of "GIPC1/NIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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